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对导入乙酰乳酸合成酶突变基因als及来自大肠杆菌的胆碱脱氢酶基因betA的棉花纯合株系T3、T4代植株的田间农艺学性状和基因漂流进行了研究。结果表明,与野生型对照鲁棉研19相比,转betA/als基因棉花涉及生存竞争力的一些农艺学性状,如种子繁殖能力、贮存后活力以及盐碱地条件下的经济产量等显著提高,转基因棉花的棉花纤维马克隆值下降,其它农艺学性状无明显差别;获得的转基因性状之一除草剂抗性遗传稳定,并在田间表达良好;在转基因棉花释放区面积为6 m×6 m的条件下,als基因通过花粉介导进入野生型棉花的频率随着非转基因棉花种植区与释放区之间的距离增大而迅速降低,采取适当的隔离距离(大于200 m),可以避免外源基因逃逸事件的发生。但设置隔离区时应考虑昆虫对花粉传播等不确定因素的影响,适当加大隔离距离。
Field agronomic traits and gene drifting of cotton homozygous T3 and T4 plants which introduced the gene als of acetolactate synthase and betA of choline dehydrogenase gene from Escherichia coli were studied. The results showed that compared with the wild-type control, Luliangyan 19, some agronomic traits such as seed multiplication ability, post-storage vitality and economic yield under salt-alkali conditions were significantly increased in turn-betA / als transgenic cotton. Transgenic The cotton fiber micronaire value of cotton decreased, and other agronomic traits had no significant difference. The herbicide resistance of one of the transgenic traits obtained was genetically stable and well expressed in the field. In the field of cotton release area of 6 m × 6 m , The frequency of als gene mediated by pollen into wild-type cotton decreased rapidly with the increase of the distance between the non-transgenic cotton planting area and the release area. The appropriate isolation distance (more than 200 m) can avoid foreign gene Escape occurred. However, the setting of isolation zone should consider insects on pollen transmission and other uncertainties, the appropriate increase in isolation distance.