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周围神经损伤后,其神经元的存活及轴突的再生有赖于多种神经经营养因子.近年来的研究显示睫状神经节营养因子(CNTF)能刺激受损的轴突生长,对神经损伤的修复起着重要作用.神经横断后,其远侧端发生Wallerian溃变,在这一时期中,CNTF在损伤变性的神经组织中能否得以表达?本实验用鼠坐骨神经损伤模型,取10例鼠坐骨神经损伤后2个月的近、远侧端神经为材料,冰冻切片,用抗CNTF进行免疫组化反应,并用IBAS Rel 20图像分析仪测量阳性免疫反应物的面积和灰度.正常组用未损伤鼠坐骨神经,并设吸收实验和置换实验.结果正常组阳性反应而
Peripheral nerve injury, the survival of neurons and axonal regeneration depends on a variety of neurotrophic factors.In recent studies have shown that ciliary ganglion nutrition factor (CNTF) can stimulate impaired axonal growth, nerve damage Of the repair plays an important role in nerve transection, the distal end Wallerian degeneration occurs, during this period, CNTF in degeneration of nerve tissue can be expressed in this experiment sciatic nerve injury model, take 10 cases The proximal and distal apices of the sciatic nerve 2 months after injury were made into frozen paraffin sections and immunohistochemically stained with anti-CNTF, and the area and the gray scale of the positive immunoreactive substances were measured with an IBAS Rel 20 image analyzer. The rat sciatic nerve was not damaged, and the absorption test and the displacement test were set up.Results The positive reaction in normal group