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本文建立了从血浆中分离白蛋白的沉淀分离法和白蛋白中S一苯基半眈氨酸(SPC)加合物的测定方法。该方法成功地用于染毒大鼠及苯接触工人的白蛋白加合物的测定。白蛋白的血浆沉淀分离;人血或大鼠血用EDTA抗凝后离心取得血浆,于血浆中加入0.5 mol/L CaCI。常温放置过夜后离心,弃去沉淀物、于*清液中加入4倍体积的生理盐水,9倍体积的酸化乙醇,于30℃温孵30 min,离心,留取上洁液;按1:ic的比例加入含有0.2mol几醋酸钠po] 95%乙醇溶液,放置15。nin,
In this paper, a method for the separation of albumin from plasma and the determination of S-phenyl-hemicyclopentylamine (SPC) adduct in albumin has been established. This method was successfully used for the determination of albumin adducts in exposed rats and benzene exposed workers. Albumin plasma sedimentation; human blood or rat blood after anticoagulation with EDTA to obtain plasma, added to the plasma 0.5 mol / L CaCI. Placed at room temperature overnight after centrifugation, discard the sediment, * 4 times the volume of the supernatant of normal saline, 9 volumes of acidified ethanol, incubated at 30 ℃ for 30 min, centrifuged, leaving the cleaning solution; press 1: ic ratio added to contain 0.2mol sodium acetate po] 95% ethanol solution, place 15. nin,