照射及给抗放药物“225”对小鼠脾超微结构的改变

来源 :中国人民解放军军事医学科学院院刊 | 被引量 : 0次 | 上传用户:dvdwen
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
应用电子显微镜对正常和经受700拉德照射及给“225”后再照射的小鼠脾超微结构变化进行了观察。经辐射损伤以后,鼠脾无论是白髓或者红髓的超微结构都出现较明显的改变,其中以淋巴细胞的变化出现较早也较严重。照后7小时为损伤时相,淋巴细胞显示不同程度的变性和坏死,轻者核染质有凝集,线粒体肿胀,基质暗淡,嵴消失等变化,重者淋巴细胞的微细结构全部消失,呈现电子致密的均质样团块状结构。此外,网状细胞、浆细胞也有变化。吞噬细胞极度活跃,胞体内吞噬的内容繁多,包括整个细胞、细胞碎片、髓样结构小体、脂滴……等。照后24小时淋巴细胞的微细结构变化仍然十分显著,吞噬细胞中所含细胞碎片等亦很多,但吞噬的高峰已经过去。照后4天,部分淋巴细胞的核仁甚为著目,出现核内包涵体。网状细胞的个别线粒体有变化,大、小溶酶体著明。窦壁内皮细胞肿胀,线粒体聚集,嵴溶解并空泡化,基底膜有裸露。照后7天,呈明显的恢复征象,白髓淋巴细胞的数量和脾索的粒、红细胞灶都增多。但即使是属恢复时相的细胞,部分仍可见微细结构的变化,如线粒体的膜增厚、嵴消失等。比较了照射鼠与给“225”后再照射的两组小鼠脾超微结构的变化,发现存在着差别。给“225”组照后7小时吞噬细胞的数量和吞噬的内容物比照射对照组为多,坏死细胞的迅速被清除可为新细胞的再生准备了条件。给“225”组照后7天脾的造血细胞灶明显的优于对照组。因此,从超微结构的水平上证明了“225”对造血组织脾是有辐射防护作用的。 The changes of the ultrastructure of spleen of mice that were exposed to 700 Radian and re-irradiated after “225” were observed by electron microscope. After radiation injury, the spleen of either white pulp or red pulp showed obvious changes in the ultrastructure, of which the change of lymphocytes appeared earlier and more serious. Seven hours after irradiation, the lymphocytes showed different degrees of degeneration and necrosis. The slight nuclear chromatin was aggregated, the mitochondria were swollen, the matrix was dark and the ridges disappeared. The fine structures of lymphocytes were all disappeared, Dense homogeneous mass of massive structure. In addition, reticulocytes, plasma cells also change. Phagocytic cells are extremely active, phagocytic content of a wide range of cells, including the entire cell, cell debris, medullary structure bodies, lipid droplets ... and so on. Twenty-four hours after irradiation, the changes in the microstructure of lymphocytes are still very significant, phagocytes contain many cellular debris, but the peak of phagocytosis has passed. 4 days after irradiation, the nucleoli of some lymphocytes are very prominent, and nuclear inclusions appear. Reticulocyte individual mitochondrial changes, large and small lysosomes with bright. Sinusoidal endothelial cell swelling, mitochondria aggregation, cristae dissolved and vacuolization, the basement membrane exposed. 7 days after irradiation, showed obvious signs of recovery, the number of white pulp lymphocytes and spleen cord granulation, red blood cells are increased. But even those cells that are in the phase of recovery can still see some changes in the microstructure, such as thickening of mitochondria and disappearance of ridges. The changes in the ultrastructure of the spleen of mice irradiated with “225” and then irradiated with “225” were compared and found to be different. The number of phagocytosed cells and the phagocytosed contents were more in the 7th hour after irradiation than in the “225” group than in the irradiated control group. The rapid elimination of necrotic cells provided the conditions for the regeneration of new cells. Seven days after giving “225” group, the hematopoietic foci of spleen were obviously better than the control group. Therefore, it is demonstrated from the ultrastructure level that “225” has a radioprotective effect on the hematopoietic tissue spleen.
其他文献
忽然想到,朋友中一些当初堪称模范的婚姻,现在几乎硕果无存了。我不由得为之唏嘘,恍然觉得普天下的婚姻都处在风雨飘摇之中。婚姻如何能长久,实在是令现代人大伤脑筋的难题
期刊
胶片本身一般能够承受机械的损伤,然而由于印片用的底片需要通过照相机、暗盒并要进行显影、水洗、干燥的过程才能完成,故会经常产生划伤。轻划伤可以通过表面填封的办法修
彩色底片是制取彩色正片的中间介质。为此,必须通过正确加工,使彩底获得适当的密度和良好的彩色平衡。由于它不能象黑白胶片那样加工时可以调整,所以最好从配制加工液到加工
彩色显影的反应历程,经过人们不懈的努力,现在大体上已经搞清楚了。但是,对于彩色显影中的染料隐色体,虽然越来越多的人相信它的存在,却一直未能得到清楚的证明。很早以前人
人类性欲的唤起,主要依赖于心理性性刺激和躯体性性刺激。心理性性刺激主要为与色情有关的文字或音像材料,它们通过视觉、听觉引起性兴奋。躯体性性刺激系用手、口、生殖器
端着丝点染色体之间的易位有数种类型,它们是人类最常见的结构畸变。这类易位中的大多数是Robertson(着丝点融合)易位,而串联易位或倒置串联易位(后者可由两端的随体而识别)
从民间立场表彰一些基层政府的创新,是为了让更多的基层政府明白,即使在现在的政治框架下,如果他真心想为老百姓办实事也还是大有可为的。 To honor some grassroots govern
不同种类的疟原虫宿主的疟疾获得性免疫力的临床形成差异很大。在有些情况下,如恒河猴感染诺氏疟原虫(Plasmodiumknowlesi)后,没有有效的免疫反应而迅速 The clinical mani