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从苹果样品中分离到苹果茎沟病毒（ＡＳＧＶ），汁液摩擦接种该病毒可侵染昆诺藜（Ｃｈｅｎｏｐｏｄｉｕｍｑｕｉｎｏａ）、苋色藜（Ｃ．ａｍａｒａｎｔｉｃｏｌｏｒ）、心叶烟（Ｎｉｃｏｔｉａｎａｇｌｕｔｉｎｏｓａ）、灰藜（Ｃ．ｎｉｕｒａｌｅ）、菜豆（Ｐｈａｓｅｏｌｕｓｖｕｌｇａｒｉｓ＂ｐｉｎｔｏ＂）和西方烟（Ｎ．ａｃｃｉｄｅｎｔａｌｉｓ＂３７Ｂ＂）。在昆诺藜叶片汁液中，该病毒体外存活期为３ｄ左右（２５℃），热钝化点６０～６５℃（１０ｍｉｎ），稀释限点１０－４。提纯病毒在－２０℃下，存活期为６个月以上。提纯病毒液Ａ２６０／Ａ２８０＝１．１８。病毒粒子呈线状，大小为６２０～６８０ｎｍ×１１～１２ｎｍ。衣壳蛋白分子量为３１．０ｋＤ。用提纯病毒免疫大耳白兔，所获抗血清用于检测提纯病毒样品和感染ＡＳＧＶ的昆诺藜叶片，其效价毛细管微量沉淀反应法为１∶５１２，酶联免疫吸附法为１∶１０４以上。用该抗血清可检测出感染ＡＳＧＶ的苹果试管苗和田间生长植株叶片。
Apple stem grooving virus (ASGV) was isolated from apple samples. The juice was rubbed and inoculated with the virus, which could infect Chenopodium quinoa, C. amaranticolor, Nicotianaglutinosa and C. quinoa. niurale, Phaseolus vulgaris pinto and N. acidentalis 37B. In Quinoa leaf juice, the virus in vitro survival of about 3d (25 ℃), heat passivation point 60 ~ 65 ℃ (10min), the dilution limit of 10-4. Purified virus at -20 ℃, the survival period of 6 months or more. Purified virus solution A260 / A280 = 1.18. Virus particles were linear, the size of 620 ~ 680nm × 11 ~ 12nm. The capsid protein has a molecular weight of 31.0 kD. The rabbits were immunized with the purified virus, and the obtained antiserum was used to detect the purified virus samples and the leaves of quinoa infected with ASGV. The titer of the antisera was 1: 512 by capillary sedimentation reaction and 1:104 by enzyme-linked immunosorbent assay the above. The antisera was used to detect ASGV-infected apple plantlets and field-grown leaves.