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目的观察诱导型环加氧酶(COX-2)抑制剂对大鼠局灶性脑缺血再灌注损伤(CIRI)的保护作用,探讨其可能机制。方法线栓法制备大鼠大脑中动脉闭塞2h/再灌注24h模型,治疗组在再灌注同时灌胃给予罗非昔布(Rofe-coxib)2.24mg/kg,对照组给予等容量生理盐水。免疫组化法测定脑组织中COX-2表达情况,用软件计算脑梗死面积,测定脑组织NO含量及NOS活性变化。结果 CIRI后脑组织COX-2表达增加,Rofecoxib 2.24mg/kg可有效抑制COX-2活性,能明显缩小脑梗死灶,降低NOS活性及NO含量。结论 Rofecoxib对大鼠局灶性脑缺血/再灌注损伤有保护作用。
Objective To investigate the protective effect of inducible cyclooxygenase (COX-2) inhibitor on focal cerebral ischemia-reperfusion injury (CIRI) in rats and its possible mechanism. Methods The middle cerebral artery occlusion (2h) / reperfusion (24h) model was established by thread occlusion. The rats in the treatment group were given Rofe-coxib (2.24mg / kg) by intragastric administration while the control group was given the same volume of normal saline. The expression of COX-2 in brain tissue was measured by immunohistochemistry. The area of cerebral infarction was calculated by software and the content of NO and the activity of NOS in brain tissue were determined. Results The expression of COX-2 in brain tissue increased after CIRI. Rofecoxib 2.24mg / kg could effectively inhibit the activity of COX-2, reduce cerebral infarction, decrease NOS activity and NO content. Conclusion Rofecoxib has a protective effect on focal cerebral ischemia / reperfusion injury in rats.