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为从鱼油中分离高纯度二十碳五烯酸 (EPA)和二十二碳六烯酸 (DHA) ,根据分子间的作用力与分子结构的关系 ,以及高效液相色谱 (HPL C)分离物质的原理 ,利用二乙烯苯交联的聚甲基丙烯酸甲酯 (PMDVB)树脂微球作为HPL C固定相 ,选择适当的流动相 ,从鱼油中分离 EPA,DHA,得到较好的分离效果。作者探索了微悬浮法合成PMDVB树脂的合成条件 ,并研究了交联度的大小和致孔剂组成对分离效果的影响。交联度为 35 % ,并具有特定孔结构的 PMDVB树脂微球作为 HPL C固定相 ,具有较高的分离度
In order to separate high-purity eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) from fish oil, according to the relationship between molecular forces and molecular structure, and high performance liquid chromatography (HPL C) Material principle, PMDVB resin microspheres crosslinked with divinylbenzene were used as HPL C stationary phase, and the appropriate mobile phase was selected to separate EPA and DHA from fish oil to obtain a better separation effect. The authors explored the synthesis conditions of PMDVB resin synthesized by micro-suspension method and studied the influence of the degree of cross-linking and the composition of porogen on the separation efficiency. The cross-linking degree of 35%, and with specific pore structure of PMDVB resin microspheres as HPL C stationary phase, with higher resolution