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目的:探讨绿原酸(CA)对小鼠海马一氧化氮合酶神经元的保护作用及改善学习记忆障碍的机理。方法:在小鼠海马定位注射海人酸(KA)建立动物病理模型,分为KA组、对照组、CA组(术后第二天开始灌胃,CA 8g/kg/d,连续35 d),用Y型迷宫测试小鼠学习记忆能力,免疫组化方法观察脑内海马NOS神经元的变化。结果:KA组比对照组小鼠海马CA1-4区内的NOS神经元明显减少(P<0.05);CA组较KA组小鼠海马CA1-4区内的NOS神经元明显增加(P<0.05);CA组较KA组小鼠在Y型迷宫中的正确次数增多。结论:CA对KA所致海马CA1-4区内的NOS神经元损害有保护作用,并改善KA损伤海马所致的学习记忆障碍。
Objective: To investigate the protective effect of chlorogenic acid (CA) on nitric oxide synthase (NOS) neurons in hippocampus and the mechanism of learning and memory impairment in mice. Methods: Animal models were established by injecting kainate into the hippocampus of mice. The animals were divided into KA group, control group and CA group (CA 8g / kg / d for 35 days) The learning and memory ability of mice was tested by Y-maze. The changes of NOS neurons in the hippocampus were observed by immunohistochemistry. Results: Compared with the control group, the number of NOS neurons in hippocampal CA1-4 region was significantly decreased in KA group (P <0.05); the number of NOS neurons in hippocampal CA1-4 region of CA group was significantly increased (P <0.05 ); CA group than KA mice in the Y-maze the correct number increased. CONCLUSION: CA has a protective effect on NOS neurons damage induced by KA in hippocampal CA1-4 region and improves learning and memory impairment induced by KA in hippocampus.