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人类染色体8p22-p21.3是前列腺癌常见的基因缺失区,提示在该区域内可能存在1个或多个抑癌基因。Fujiwara等曾在此区域分离到一个命名为PRLTS的候选基因(血小板衍化生长因子受体样抑癌基因)。作者通过LOH、PCR-SSCP和直接测序等方法探讨了前列腺癌组织中PRLTS基因的变化情况。共分析165份组织标本,包括69例前列腺癌组织、69例正常组织和27例尸检时从肝、肺和盆腔淋巴结获得的转移癌组织。
Human chromosome 8p22-p21.3 is a common gene deletion region in prostate cancer, suggesting that there may be one or more tumor suppressor genes in this region. Fujiwara et al. isolated a candidate gene named PRLTS (platelet-derived growth factor receptor-like tumor suppressor gene) in this region. The authors explored the changes of PRLTS gene in prostate cancer tissues by LOH, PCR-SSCP and direct sequencing. A total of 165 tissue samples were analyzed, including 69 prostate cancer tissues, 69 normal tissues, and 27 metastatic cancer tissues obtained from liver, lung, and pelvic lymph nodes at autopsy.