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目的探讨在高原环境下建立高原肺水肿(HAPE)动物模型的方法。方法将大白兔随机分为对照组(N)、地塞米松组(D)、普萘洛尔组(P)、睡眠干扰组(G),在海拔3700 m高原冬季环境下,给动物以13.3 ml/min速度输37℃生理盐水200 ml,20 min后再输200 ml,共输400 ml。试验中观察动物血压、中心静脉压、呼吸、心率等情况。结果 G组3只发生HAPE,P组2只,而N组和D组没有发生;G组的肺含水量比N组明显增高(P<0.01),P、D组肺含水量比N组减少,但差异无统计学意义。结论在高原现场,通过输液诱导方式,在药物影响肺重吸收功能和睡眠干扰情况下,复制出了肺泡型HAPE模型。
Objective To explore a method for establishing animal model of high altitude pulmonary edema (HAPE) in the plateau environment. Methods The rabbits were randomly divided into control group (N), dexamethasone group (D), propranolol group (P) and sleep disturbance group (G). Under the winter environment of 3700 m altitude, ml / min speed of 37 ℃ saline 200 ml, 200 ml after 20 min, a total of 400 ml. The experiment observed animal blood pressure, central venous pressure, respiration, heart rate and so on. Results There were 3 HAPE group and 2 P group in G group, while N group and D group did not occur. The lung water content of G group was significantly higher than that of N group (P <0.01) , But the difference was not statistically significant. Conclusion In altiplano field, the alveolar type HAPE model was replicated by the induction of infusion in the condition of drug affecting pulmonary reabsorption and sleep disturbance.