宫血灵为动物之蹄甲类药物,主治功能性子宫出血及月经过多。作者对宫血灵中氨基酸的定量、含量测定进行了探讨。 1 样品制备 1.1 精确称取宫血灵粉50mg,加入适量6NHCl火焰封管,于110℃消化22—24小时,制成水解液。 1.2 精确称取宫血灵片研磨粉末50mg,消化方法同上。 1.3 宫血灵原测定方法的制备是按以下两种方法制备:(1)凯氏定氮法;(2)双向纸层析。 Gong Xue Ling is an animal’s trotters, and it treats functional uterine bleeding and menorrhagia. The authors discussed the amino acid quantification and content determination in Gongxueling. 1 Sample preparation 1.1 Accurately weigh 50 mg of Gongxueling powder, add an appropriate amount of 6N HCl flame to seal the tube, and digest it at 110°C for 22-24 hours to make a hydrolyzate. 1.2 Accurately weighed 50mg of Gongxueling tablet grinding powder. The digestion method is the same as above. 1.3 The preparation of the method for the determination of glycopyrrolate is prepared according to the following two methods: (1) Kjeldahl method; (2) Bidirectional paper chromatography.