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PA_C subunit from avian influenza(H5N1) viral RNA polymerase was used in this work as a target in the screening for anti-influenza agents from licorice-derived compounds.As a result,18β-glycyrrhetinic acid was suggested to be PA_C ligand by flexible docking,and was then confirmed by relaxation-edited NMR.The result of ApG primer extension assay indicated that this PA_C ligand can inhibit the polymerase activity,and thus may potentially be valuable as anti-influenza lead compound.This work validated the possibility of screening polymerase inhibitors by using PA_C as a target,and provided a starting point for the further discovery of new anti-influenza drugs.
PA_C subunit from avian influenza (H5N1) viral RNA polymerase was used in this work as a target in the screening for anti-influenza agents from licorice-derived compounds. As a result, 18β-glycyrrhetinic acid was suggested to be PA_C ligand by flexible docking , and was then confirmed by a relaxed-compiled NMR. The result of the ApG primer extension assay indicated that this PA_C ligand can inhibit the polymerase activity, and thus may potentially be valuable as anti-influenza lead compound. This work validated the possibility of screening for the polymerase inhibitors by using PA_C as a target, and provide a starting point for the further discovery of new anti-influenza drugs.