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观察环孢菌素A(CsA)能否阻断刀豆蛋白A (ConA)诱发小鼠肝损害 ,将昆明小鼠随机分为 5组 ,ConA自小鼠尾静脉注入制作肝损害实验动物模型 ,据ConA应用剂量不同而分别称为ConA 10mg/kg组 ,ConA 2 0mg/kg组和ConA 40mg/kg组 ;应用ConA前 15h和 1h皮下注射CsA(130mg/kg) ,该组称为CsA组 ;单独用PBS者为对照组。应用ConA后 8h取小鼠血测定ALT与TNF α ,并取肝组织行病理学观察。结果显示 ,ConA成功复制了小鼠特异性肝损害 ;CsA组小鼠血浆中ALT与PBS组相比无显著性差异 (P >0 .0 5 ) ,血浆TNF α亦无增高 ;病理学观察未发现肝脏内淋巴细胞及其他炎性细胞浸润。提示CsA可完全阻断ConA诱发小鼠肝损害 ,抑制T淋巴细胞活化和TNF α的释放是其重要的保护机制
To observe whether cyclosporine A (CsA) could block ConA-induced liver damage in mice, Kunming mice were randomly divided into 5 groups. ConA was injected into the caudal vein of mice to make liver damage experimental animal model, ConA 10 mg / kg group, ConA 2 0 mg / kg group and ConA 40 mg / kg group according to different dosage of ConA; CsA group (130mg / kg) was injected subcutaneously 15h and 1h before ConA group, PBS alone as a control group. 8h after application of ConA, the blood of mice was taken to determine ALT and TNFα, and the pathological changes of liver were observed. The results showed that ConA successfully replicated mouse-specific liver damage. There was no significant difference in plasma ALT between CsA group and PBS group (P> 0.05), and plasma TNFα also did not increase. Pathological observation did not Found in the liver lymphocytes and other inflammatory cell infiltration. It suggested that CsA can completely block ConA-induced liver damage, inhibit the activation of T lymphocytes and release of TNFα, which is an important protective mechanism