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研究芍药内酯苷、芍药苷对慢性束缚应激肝郁大鼠的作用及机制。以氟西汀及逍遥丸为对照,检测体重,糖水消耗量,并进行旷场行为学实验;格瑞斯比色法检测海马一氧化氮(NO)的含量;酶免法(ELISA)检测海马脑源性神经营养因子(BDNF)的含量;RT-q PCR法检测海马神经元一氧化氮合酶(n NOS)m RNA表达;Western blot法检测海马中n NOS以及BDNF的蛋白表达。与模型组相比,30 mg·kg~(-1)芍药苷、30 mg·kg~(-1)芍药内酯苷明显升高穿行次数(P<0.05;P<0.01);芍药苷、芍药内酯苷明显降低NO水平(P<0.05,P<0.01;P<0.05,P<0.05);30 mg·kg~(-1)芍药苷与30 mg·kg~(-1)芍药内酯苷明显升高BDNF水平(P<0.05)。与模型组相比,芍药苷、芍药内酯苷明显降低n NOS m RNA表达(P<0.01;P<0.05);30 mg·kg~(-1)芍药苷、30 mg·kg~(-1)芍药内酯苷明显升高BDNF蛋白表达(P<0.05)。芍药内酯苷、芍药苷为白芍柔肝解郁功效的有效成分,其作用机制与调节海马NO含量以及BDNF的表达有关。
To study the effect and mechanism of paeoniflorin and paeoniflorin on chronic stress-induced liver depression in rats. Fluoxetine and Xiaoyao Pill were used as control to test body weight and sugar consumption, and open-field behavioral tests were performed. The content of nitric oxide (NO) in the hippocampus was detected by Griess colorimetric assay. The content of BDNF was detected by RT-qPCR method. The expression of nitric oxide synthase (nNOS) m RNA in hippocampal neurons was detected by RT-q PCR. The protein expression of nNOS and BDNF in hippocampus was detected by Western blot. Compared with the model group, the paeoniflorin 30 mg · kg -1 and the peony lactone 30 mg · kg -1 significantly increased the number of passages (P <0.05; P <0.01) Lactone significantly reduced the level of NO (P <0.05, P <0.01; P <0.05, P <0.05), and 30 mg · kg -1 paeoniflorin and 30 mg · kg -1 paeoniflorin Significantly increased the level of BDNF (P <0.05). Compared with the model group, paeoniflorin and paeoniflorin significantly decreased the expression of nNOS m RNA (P <0.01; P <0.05), while those of 30 mg · kg -1 paeoniflorin and 30 mg · kg -1 ) Paeoniflorin significantly increased BDNF protein expression (P <0.05). Paeoniflorin and paeoniflorin are the effective components of Rhizoma Paridis Rouxueyin and its mechanism may be related to the regulation of NO content in the hippocampus and the expression of BDNF.