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目的:探讨miRNA-26a在支气管哮喘患儿及支气管哮喘小鼠模型中的表达。方法:应用real-time PCR法检测支气管哮喘患儿外周血淋巴细胞中miRNA-26a与正常儿童的表达差异。采用卵清蛋白(ovalbumin,OVA)诱导支气管哮喘小鼠模型,通过检测肺泡灌洗液(BALF)中细胞数及组织病理验证模型建立。应用real-time PCR法检测支气管哮喘小鼠模型肺组织miRNA-26a与对照组的表达差异。采用Western blot法检测小鼠肺组织GSK-3β的表达差异。结果:支气管哮喘患儿外周血淋巴细胞miRNA-26a显著高于正常对照组。OVA诱导的支气管哮喘小鼠模型组BALF中细胞总数及嗜酸性粒细胞数显著高于正常对照组。组织病理显示支气管哮喘模型组炎症细胞浸润明显高于正常对照组。支气管哮喘小鼠模型组肺组织miRNA-26a显著高于正常对照,GSK-3β蛋白水平明显低于正常对照组。结论:miRNA-26a在支气管哮喘患儿及支气管哮喘小鼠模型中高表达。
Objective: To investigate the expression of miRNA-26a in bronchial asthma and bronchial asthma mouse model. Methods: Real-time PCR was used to detect the difference of miRNA-26a and normal children’s lymphocytes in children with bronchial asthma. A mouse model of bronchial asthma was induced by ovalbumin (OVA), and the number of cells in the BALF and the histopathological verification model were established. Real-time PCR was used to detect the difference of miRNA-26a expression in the lung tissue of the bronchial asthma mouse model and the control group. Western blot was used to detect the difference of GSK-3β expression in mouse lung tissue. Results: The levels of miRNA-26a in peripheral blood lymphocytes in children with bronchial asthma were significantly higher than those in normal controls. The total number of BALF cells and the number of eosinophils in OVA-induced bronchial asthma mouse model group were significantly higher than those in normal control group. Histopathology showed that infiltration of inflammatory cells in bronchial asthma model group was significantly higher than that of the normal control group. The expression of miRNA-26a in bronchial asthma model group was significantly higher than that in normal control group, and the level of GSK-3β protein in asthma model group was significantly lower than that in normal control group. Conclusion: miRNA-26a is highly expressed in asthmatic mice and asthmatic mice.