Objective To detect the content of total flavonoids in different plant parts of five species of HERBA MOGHANIAE in different grown areas; to evaluate the qualities of different species of medicinal materials in different grown areas; and to find out new medicinal resources and medicinal plant parts.Methods The content of total flavonoids was detected at 510 nm by UV-Vis spectrophotometry.Results Rutin showed good linear relationship between 9.17 and 64.19 μg/mL; the average recovery rate was 99.63% and RSD was 1.36%(n=9).Among the different species,contents of total flavonoids in the roots of Moghania strobilifera (Linn.)Ait.and M.latifolia Benth.were lower than those of M.philippinensis (Merr.et Rolfe)Li and M.macrophylla (Willd.) O.Kuntze.Total flavonoids content in the roots of M.glutiaosa (Prain)Y.T.Wei was approximated to those in the roots of M.philippinensis (Merr.et Rolfe)Li and M.macrophylla (Willd.) O.Kuntze.Total flavonoids contents in the leaves of different species were relatively high,but those in the stems were relatively low.As for the plants in different grown areas,M.macrophylla (Willd.) O.Kuntze purchased from Chengdu City of Sichuan Province (grown in Yunnan Province) had the maximum content; M.philippinensis (Merr.et Rolfe) Li grown in Yiling District of Yichang City of Hubei Province had the maximum content.Total flavonoids contents in M.strobilifera (Linn.)Ait.in different grown areas were all relatively low.Conclusions This method was simple,rapid and accurate,and could be used for the quality control of HERBA MOGHANIAE.There were great differences in the total flavonoids contents in HERBA MOGHANIAE in different grown areas,indicating that altitude,climate,soil condition,growth years and storage years had significant impacts on the total flavonoids content in HERBA MOGHANIAE.With respect to the total flavonoids,the effective and sustainable utilization of resources,it was suggested that the leaves and stems of different species of HERBA MOGHANIAE could be used as medicines together with roots; and the stems could also be used.M.glutiaosa (Prain)Y.T.Wei could also be used as a alternative medicinal resource. Objective To detect the content of total flavonoids in different plant parts of five species of HERBA MOGHANIAE in different grown areas; to evaluate the qualities of different species of medicinal materials in different grown areas; and to find out new medicinal resources and medicinal plant parts. Methods The content of total flavonoids was detected at 510 nm by UV-Vis spectrophotometry. Results Rutin showed good linear relationship between 9.17 and 64.19 μg / mL; the average recovery rate was 99.63% and RSD was 1.36% (n = 9). Among the different species, contents of total flavonoids in the roots of Moghania strobilifera (Linn.) Ait. and M. latifolia Benth. lower than those of M. philippinensis (Merr.et Rolfe) Li and M. macrophylla (Willd.) O .Kuntze.Total flavonoids content in the roots of M.glutiaosa (Prain) YTWei approximated to those in the roots of M.philippinensis (Merr.et Rolfe) Li and M.macrophylla (Willd.) O.Kuntze.Total flavonoids contents in the leaves of different species were r elatively high, but those in the stems were relatively low. As for the plants in different grown areas, M. macrophylla (Willd.) O. Kuntze purchased from Chengdu City of Sichuan Province (grown in Yunnan Province) had the maximum content; M . Philippinensis (Merr.et Rolfe) Li grown in Yiling District of Yichang City of Hubei Province had the maximum content. Total flavonoids contents in M. strobilifera (Linn.) Ait. in different grown areas were all relatively low. Confclusions This method was simple, rapid and accurate, and could be used for the quality control of HERBA MOGHANIAE.There were great differences in the total flavonoids contents in HERBA MOGHANIAE in different grown areas, indicating that altitude, climate, soil condition, growth years and storage years had significant impacts on the total flavonoids content in HERBA MOGHANIAE. Whilst respect to the total flavonoids, the effective and sustainable utilization of resources, it was suggested that the leaves and stems of different species of HERBA MOGH ANIAE could be used as medicines together with roots; and the stems could also be used. M. gratiaosa (Prain) Y.T.Wei could also be used as a alternative medicinal resource.