一、显色反应的灵敏度和胶束增溶分光光度法在分光光度法中,最基本的规律是Lambert-Beer定律,即称为克分子吸收系数。ε数值的大小,通常是显色反应灵敏度的标志,ε值越大,表示灵敏度越高。过去,人们没有从理论上搞清ε的本质,常企图借实验方法的改进,无限地提高值。直到1950年,有人研究了光吸收度和分子截面积之间的关系,得到下列关系式:式中〔ε〕=理论上可能达到的最大的克分子吸收系数; 〔a〕=显色分子的平均截面积。对于简单分子来说,〔a〕通常为10埃~2数量级,亦即10~(-15)cm~2,代入上式,公式(2)不仅从理论上阐明了显色反应可 First, the sensitivity of color reaction and micellar solubilization spectrophotometry Spectrophotometry, the most basic rule is Lambert-Beer’s law, known as the molecular absorption coefficient. The value of ε is usually a sign of the color reaction sensitivity. The larger the value of ε, the higher the sensitivity. In the past, people did not theoretically find the essence of ε, often trying to borrow experimental method improvements, infinitely increase value. It was not until 1950 that the relationship between light absorbance and molecular cross-sectional area was studied to obtain the following relationship: where [epsilon] = theoretically the maximum possible molecular absorption coefficient; [a] = Average cross-sectional area. For simple molecules, [a] is usually on the order of 10 angstroms to 2 orders of magnitude, ie, 10 to (-15) cm ~ 2 and is substituted into the above formula. Equation (2) not only clarifies the chromogenic reaction