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背景:现代医学发现通心络制剂除了具有抗凝和抑制血小板聚集作用外,对血管内皮细胞有一定的保护作用。目的:观察中药复方制剂通心络是否影响脑缺血再灌注动物模型黏附分子的表达。设计:随机对照实验。单位:解放军第二军医大学长征医院神经内科。材料:实验于2002-10/2003-01在解放军第二军医大学长征医院神经内科实验室完成。选择雄性SD大鼠25只,随机分为假手术组5只、模型组10只和通心络组10只。方法:线栓法制备大鼠大脑中动脉脑局灶性脑缺血再灌注模型,假手术组除将尼龙线插在颈外动脉接近颈内动脉分叉处外,其余同模型组。通心络组大鼠在缺血再灌注前给予通心络粉剂1.0g/(kg·d),溶在生理盐水中灌胃1周。模型组和假手术组灌胃等剂量生理盐水。各组大鼠麻醉后取脑制备切片,行常规苏木精-伊红染色、免疫组化及原位杂交染色。主要观察指标:①缺血再灌注后细胞间黏附分子1和血管细胞黏附分子1阳性微血管表达数目。②缺血再灌注后细胞间黏附分子1mRNA阳性微血管表达数目。结果:①假手术组手术侧大脑半球皮质和基底节区未见细胞间黏附分子1、血管细胞黏附分子1蛋白和细胞间黏附分子1mRNA阳性微血管表达。②模型组大鼠缺血2h再灌注6h后,缺血侧大脑细胞间黏附分子1、血管细胞黏附分子-1蛋白表达水平和细胞间黏附分子1mRNA表达水平显著升高。③通心络组缺血侧大脑半球皮质和基底节区蛋白和mRNA阳性微血管数较模型组显著降低犤(10.42±1.98),(12.42±2.14)/高倍视野;(8.54±2.00),(11.12±1.56)/高倍视野犦(P<0.05),血管细胞黏附分子1蛋白阳性微血管表达数目无显著变化(P>0.05)。结论:通心络可以降低大鼠脑缺血再灌注后细胞间黏附分子1的转录和翻译过程,有助于减轻脑缺血后的炎症性损伤过程。
BACKGROUND: Modern medicine found that Tongxinluo preparation has a protective effect on vascular endothelial cells in addition to anticoagulation and inhibition of platelet aggregation. Objective: To observe whether Tongxinluo, a traditional Chinese medicine preparation, affects the expression of adhesion molecules in cerebral ischemia-reperfusion animal models. Design: Randomized controlled trials. Unit: Department of Neurology, Changzheng Hospital, Second Military Medical University, Chinese People’s Liberation Army. MATERIALS: The experiment was performed at the Department of Neurology, Changzheng Hospital, Second Military Medical University of Chinese PLA from October 2002 to January 2003. Twenty-five male SD rats were randomly divided into 5 sham-operated groups, 10 model groups, and 10 Tongxinluo groups. METHODS: The model of cerebral middle cerebral artery ischemic reperfusion in rats was prepared by suture embolization. The sham-operated group was inserted into the external carotid artery near the internal carotid artery bifurcation, and the other rats were in the same model group. Tongxinluo group was given Tongxinluo powder (1.0g/(kg·d)) before ischemia and reperfusion, and was dissolved in physiological saline for one week. The model group and sham group were given equal doses of normal saline. Each group of rats was anesthetized and brain sections were prepared for routine hematoxylin-eosin staining, immunohistochemistry, and in situ hybridization staining. MAIN OUTCOME MEASURES: 1 The number of positive microvessels expressed in intercellular adhesion molecule 1 and vascular cell adhesion molecule 1 after ischemia-reperfusion. 2 The number of positive microvessels expressed in intercellular adhesion molecule 1 mRNA after ischemia-reperfusion. RESULTS: 1 There was no expression of intercellular adhesion molecule 1, vascular cell adhesion molecule 1 protein and intercellular adhesion molecule 1 mRNA-positive microvessel in the sham-operated group in the cerebral hemisphere cortex and basal ganglia. 2 After 6 hours of ischemia and ischemia in the model group, the expression levels of intercellular adhesion molecule-1, vascular cell adhesion molecule-1 and intercellular adhesion molecule-1 mRNA expression were significantly increased in the ischemic side. 3 The number of protein and mRNA positive microvessels in cerebral cortex and basal ganglia of the ischemic hemispheres was significantly lower than that of the model group (10.42±1.98), (12.42±2.14)/high power field; (8.54±2.00), (11.12) ±1.56)/high power field vision (P<0.05), and the number of vascular cell adhesion molecule 1 protein-positive microvessels showed no significant changes (P>0.05). Conclusion: Tongxinluo can reduce the transcription and translation of ICAM-1 after cerebral ischemia-reperfusion in rats, which is helpful to relieve the inflammatory injury after cerebral ischemia.