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近年来许多学者逐渐认识到凋亡与肿瘤关系是当今肿瘤研究的热点,因为恶性肿瘤的产生和发展,不但是由于细胞的无限增殖,还由于细胞内部控制细胞死亡通道即凋亡(apoptosis)的机制障碍或二者失去平衡所致。我们应用流式细胞仪、电镜和琼脂糖DNA电泳,研究热疗联合MMC对诱导LoVo细胞凋亡和bcl-2基因表达的作用,并初步探讨其机制。 1 材料和方法 1.1 材料人大肠癌细胞株(LoVo)由第一军医大学病理教研室提供。丝裂霉素C为日本东京协和发酵工业株式会社产品。碘化丙锭(propidium iodide,PI)是美国Sigma公司的产品。RNA酶为中国科学院生物化学研究所产品。 1.2 方法 LoVo细胞常规贴壁于37℃,50mL·L~(-1)CO_2孵箱
In recent years, many scholars have come to realize that the relationship between apoptosis and tumors is a hot topic in current cancer research, because the development and progression of malignant tumors is not only due to the unlimited proliferation of cells, but also due to the internal control of cell death pathways, ie, apoptosis (apoptosis). Obstacles to the mechanics or the loss of balance between the two. We used flow cytometry, electron microscopy, and agarose DNA electrophoresis to study the effect of hyperthermia combined with MMC on the induction of LoVo cell apoptosis and bcl-2 gene expression, and to explore its mechanism. 1 Materials and methods 1.1 Materials Human colorectal cancer cell line (LoVo) was provided by Department of Pathology, First Military Medical University. Mitomycin C is a product of Japan’s Tokyo Concorde Fermentation Industry Co., Ltd. Propidium iodide (PI) is a product of Sigma, USA. RNase is a product of the Institute of Biochemistry of the Chinese Academy of Sciences. 1.2 Methods Conventionally, LoVo cells were adhered to 37°C, 50mL·L -1 CO 2 incubator