Dear Editor,The CRISPR/Cas gene editing system offers great potential for functional genomics in plants and crop improvement.The spec ificity of Cas-directed DNA cleavage is strictly determined by a chimeric single guide RNA (sgRNA) and a short protospacer adjacent motif (PAM) in the genome (Cong et al.,2013;Zetsche et al.,2015).The widely used SpCas9 and its variants have been shown to recognize PAM sequences in the canonical form NGG and non-canonical NGA,NAG,or NGCG in plants (Miao et al.,2013;Ma et al.,2015;Hu et al.,2016).CRISPR/Cpf1,a new class 2 CRISPR/Cas system,was recently exploited as an alteative tool for genome editing in various organisms,including plants (Zetsche et al.,2015;Kim et al.,2017;Tang et al.,2017;Wang et al.,2017;Xu et al.,2017).Cpf1 utilizes a thymidine-rich PAM site,TTTN,and is guided by a single CRISPR RNA (crRNA) (Zetsche et al.,2015).