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目的:通过滴水珠组织培养及快速繁殖,解决滴水珠的资源匮乏问题。方法:以滴水珠的叶片及叶柄为外植体,探索滴水珠愈伤组织诱导与增殖的最佳培养基,并对滴水珠愈伤组织的分化、生根诱导及组培苗的移栽进行研究。结果:滴水珠在不同浓度2,4-D与6-BA或KT的培养基上可以较好地诱导出滴水珠的愈伤组织,MS+2.0mg/L6-BA+0.2mg/L 2,4-D是滴水珠愈伤组织最佳诱导和增殖培养基;在含不同浓度NAA与6-BA的MS培养基中,滴水珠能快速生长。结论:本研究初步建立了完整的滴水珠的组织培养体系,具有开发利用潜力。
Objective: To solve the problem of lack of resources of dripping beads through drip bead tissue culture and rapid propagation. Methods: Leaf and petiole of drip bead were used as explants to explore the best culture medium for callus induction and proliferation of drip bead. The differentiation and rooting of callus and transplanting of tissue culture seedling were studied. . The results showed that the dripping beads could induce the callus of drip bead well under the different concentrations of 2,4-D and 6-BA or KT, MS + 2.0mg / L 6-BA + 0.2mg / L 2, 4-D was the best medium for inducing and proliferating drip callus. In MS medium with different concentrations of NAA and 6-BA, drip beads could grow rapidly. Conclusion: This study initially established a complete drip bead tissue culture system, with the potential for development and utilization.