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目的了解山东泰安、济南、东营三地区女性人乳头瘤病毒(HPV)各基因型别的感染和分布情况,为山东三地区HPV分子流行病学研究提供依据。方法回顾性分析2014年1月-2016年6月在山东省三地区就诊、筛查和体检的34 258例宫颈脱落细胞标本,采用PCR体外扩增和DNA反向点杂交相结合的DNA芯片技术,对宫颈脱落细胞标本进行HPV基因分型检测。结果不同地区人群HPV阳性率及高危、低危、多重感染率差异有统计学意义(P<0.01),三地区检出阳性标本:泰安地区检出阳性标本701例,占18.17%(701/3 857),其中高危型HPV 339例,占8.78%(339/3 857,包含多重感染);低危型HPV 151例,占3.91%(151/3 857,包含多重感染),多重感染297例,占7.70%(297/3 857);济南地区检出阳性标本1 825例,占17.86%(1 825/10 216),其中高危型HPV 1 486例,占14.54%(1 486/10 216,包含多重感染);低危型HPV 338例,占3.30%(338/10 216,包含多重感染),多重感染626例,占6.13%(626/10 216);东营地区检出阳性标本5 607例,占27.78%(5 607/20 185),其中高危型HPV 4 431例,占21.95%(4 431/20 185,包含多重感染);低危型HPV 1 176例,占5.83%(1 176/20 185,包含多重感染),多重感染2 167例,占10.74%(2 167/20 185)。23种型别均被检出,三地区HPV亚型前5位有所区别,各类型HPV感染顺位分别是泰安为HPV16、HPV52、HPV58、HPV53、HPV81;济南为HPV16、HPV52、HPV58、HPV6、HPV81;东营为HPV16、HPV52、HPV58、HPV18、HPV51,不同地区之间HPV感染率及各型别感染率差异有统计学意义(P<0.01)。结论各地区之间女性人群的HPV感染率及HPV型别感染率存在显著差异,因此早期筛查对控制HPV的感染是预防或降低宫颈病变的有效途径。
Objective To understand the infection and distribution of HPV genotypes in Tai’an, Jinan and Dongying, Shandong Province, and to provide evidence for the molecular epidemiology of HPV in three regions of Shandong Province. Methods A retrospective analysis of 34 258 cases of cervical exfoliated cells from January 2014 to June 2016 in three districts of Shandong Province was performed. Screening and physical examination were performed by DNA chip technique combining PCR in vitro amplification and DNA reverse dot blot hybridization , Cervical exfoliated cell specimens HPV genotyping test. Results There were significant differences in HPV positive rate, high risk, low risk and multiple infection among different regions (P <0.01). Positive samples were detected in three regions: 701 positive samples were detected in Taian area, accounting for 18.17% (701/3 857), of which 339 were high-risk HPV, accounting for 8.78% (339/3 857, multiple infections); 151 were low-risk HPV (3.91%, 151/3 857, multiple infections), 297 were multiple infections Accounting for 7.70% (297/3 857). There were 1825 positive samples (17.86%) in Jinan area (1825/1016), including 1 486 high-risk HPV types (14.54% Multiple infections); low-risk HPV 338 cases, accounting for 3.30% (338/10216, including multiple infections), multiple infections in 626 cases, accounting for 6.13% (626/10216); positive samples in Dongying 5 607 cases, Accounting for 27.78% (5 607/20 185) of whom 4343 were high risk HPVs, accounting for 21.95% (4 431/20 185, including multiple infections); 1 176 low risk HPVs (5.83% 185, with multiple infections), 2 167 multiple infections (10.74%) (2 167/20 185). 23 types were detected, the three regions of the top 5 HPV subtype differences, the various types of HPV infection rankings are Tai’an HPV16, HPV52, HPV58, HPV53, HPV81; Jinan HPV16, HPV52, HPV58, HPV6 , HPV81; HPV16, HPV52, HPV58, HPV18 and HPV51 in Dongying were statistically different (P <0.01). Conclusion There is a significant difference in HPV infection rate and HPV type infection rate between female population in different regions. Therefore, early screening is an effective way to prevent or reduce cervical lesions by controlling HPV infection.