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目的观察小鼠有机磷中毒后肌无力的情况及病理改变。方法将117只昆明种小鼠分为7个实验组(1～7 d观察组),每组15只,1个正常对照组(6只)及1个生理盐水对照组(6只)。对实验组以50 mg/kg氧化乐果腹腔注射染毒(1～7 d);正常对照组不予任何处理;生理盐水对照组给予生理盐水0.9ml腹腔注射。观察小鼠出现肌无力情况.并留取膈肌和股直肌行HE染色和乙酰胆碱酯酶(AChE)染色,观察肌肉病理改变及运动终板数目及着色变化。结果105只小鼠急性氧乐果中毒后43只出现肌无力,肌无力发生率40.1%:急性期小鼠死亡7只,死亡率为6.7%:肌无力主要表现为肢体力弱,屈颈肌无力及呼吸困难不明显。肌肉病理染色结果显示,HE染色中实验组肌无力与非肌无力小鼠镜下均呈炎性改变,表现为肌膜核增加,核内移,肌间质炎细胞浸润。肌无力小鼠的肌肉运动终板数目较对照组明显减少,差异有统计学意义(P<0.05);肌无力组膈肌2、3、7 d和股直肌1、3、5、7 d的运动终板数明显低于非肌无力组,差异有统计学意义(P<0.05);各时间点肌无力组肌肉的运动终板数差异无统计学意义(P>0.05)。结论小鼠急性氧乐果中毒后部分出现肌无力;肌无力小鼠肌肉光学显微镜下表现为非特异性炎性改变,AChE染色运动终板数目减少,但不随时间延长而减少。
Objective To observe the situation of muscle weakness and pathological changes after organophosphate poisoning in mice. Methods A total of 117 Kunming mice were divided into seven experimental groups (1-7 days), 15 rats in each group, 6 normal saline control group and 6 normal saline control group. Rats in the experimental group were given intraperitoneal injection of 50 mg / kg omethoate (1-7 d). No treatment was given in the normal control group. The saline control group was given 0.9 ml normal saline intraperitoneally. Muscle weakness was observed in mice, and the diaphragm and rectus femoris were stained with hematoxylin and eosin (AChE), and the pathological changes of muscles and the number of motile end plates were observed. RESULTS: Thirty-four mice with acute oxytoxic poisoning had muscular weakness. The incidence of myasthenia gravis was 40.1%. Seven mice died in acute phase with a mortality rate of 6.7%. Muscular weakness mainly manifested as weak limbs, Inability and difficulty breathing is not obvious. Muscle pathological staining results showed that in the HE group, the mice in the test group showed inflammatory changes in the muscles of the weak and non-weak mice, showing the increase of the sarcolemma, the shift of the nucleus and the infiltration of interstitial cells. Muscle weakness mice muscle endplate number than the control group was significantly reduced, the difference was statistically significant (P <0.05); muscle weakness group at 2, 3, 7 d and 1, 3, 5, 7 d (P <0.05). There was no significant difference in motor end plate number of muscles in muscle weakness group at each time point (P> 0.05). Conclusions Muscle weakness occurred partly in mice after acute administration of omethoate. The mice with muscle weakness showed nonspecific inflammatory changes under muscle optical microscope. The number of AChE staining motor end plates decreased but did not decrease with time.