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目的探讨大规模接种前抗体水平对甲型H1N1流行性感冒裂解疫苗(简称甲流疫苗)免疫原性和免疫持久性的影响。方法采用多阶段随机抽样方法,抽取3~5岁、6~10岁、11~15岁、16~20岁、21~40岁、41~60岁和60岁以上7个年龄组计350人作为监测对象。使用微量血凝抑制(HI)实验测定监测对象接种甲流疫苗前、接种后28 d和6个月甲流抗体水平。计算抗体平均滴度、抗体阳转率和抗体保护率,分析接种前抗体水平与抗体阳性率、抗体保护率的关系。结果接种前HI抗体平均滴度1:6.65,抗体保护率17.1%,接种后28 d和6个月甲流HI抗体平均滴度、阳转率和保护率均超过了欧盟药品评价局(EMEA)和美国FDA的标准。接种前抗体水平与免疫后28 d和6个月抗体阳转率均呈负相关,与抗体保护率均无相关性。结论甲流流行期间,为了提高保护率,保护易感人群,可以不考虑接种前抗体水平,并可取得较好的免疫原性和免疫持久性。
Objective To investigate the effect of large-scale pre-vaccination antibody on immunogenicity and immune persistence of influenza A (H1N1) Methods A total of 350 people in 7 age groups of 3-5 years old, 6-10 years old, 11-15 years old, 16-20 years old, 21-40 years old, 41-60 years old and 60 years old and above were sampled by multi-stage random sampling method Monitoring object. Fluorescent antibody levels were measured at 28 days and 6 months after inoculation with a small amount of hemagglutination inhibition (HI) assay before and after vaccination. The average antibody titers, antibody positive rate and antibody protection rate were calculated. The relationship between the antibody level before antibody inoculation and antibody positive rate and antibody protection rate was analyzed. Results The average titer of HI antibody before inoculation was 1: 6.65 and the antibody protection rate was 17.1%. The average titer, positive rate and protection rate of HI antibody at 28 d and 6 months after inoculation exceeded the EMEA, And the United States FDA standards. The antibody level before inoculation was negatively correlated with the antibody positive rate at 28 d and 6 months after immunization, and no correlation with antibody protection rate. Conclusion During the epidemic of Influenza A, in order to improve the protection rate and protect the susceptible population, the antibody level before inoculation can be considered, and better immunogenicity and immunological persistence can be obtained.