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目的了解北京地区婴幼儿病毒性腹泻的病原学特点。方法采集2011年1月-12月5岁以下腹泻患儿的粪便标本并填写个案调查表,用ELISA试剂盒检测A组轮状病毒,采用逆转录-聚合酶链反应(RT-PCR)检测人杯状病毒和星状病毒,采用PCR检测腺病毒。结果 604例粪便标本中,A组轮状病毒检出率15.89%,人杯状病毒检出率18.71%,星状病毒检出率2.98%,腺病毒检出率4.80%,病毒混合感染27例。11月份轮状病毒检出率最高,10月份人杯状病毒检出率最高。结论 A组轮状病毒和人杯状病毒为北京地区秋冬季婴幼儿腹泻的主要病原。
Objective To understand the etiological characteristics of infant diarrhea in Beijing. Methods Stool samples from children with diarrhea under 5 years of age from January to December 2011 were collected and completed. Case rotavirus was detected by ELISA kit and RT-PCR was used to detect human Caliciviruses and astroviruses were used to detect adenovirus by PCR. Results Among the 604 stool samples, the detection rate of rotavirus in group A was 15.89%, the rate of human calicivirus was 18.71%, the detection rate of astrovirus was 2.98%, the detection rate of adenovirus was 4.80%, and the virus mixed infection was 27 . The highest detection rate of rotavirus in November and the highest detection rate of human calicivirus in October. Conclusions Group A rotavirus and human calicivirus are the major pathogens of infantile diarrhea in autumn and winter in Beijing.