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目的:观察复方加味四逆散及拆方的药物血清对肝星状细胞(HSC-T6)JAK2-STAT3信号通路的影响。方法:用加味四逆散及拆方煎剂给正常Wistar大鼠灌胃7天,制备含药血清;培养肝星状细胞;用100ng/mL的瘦素刺激HSC-T6使其增殖;应用蛋白印迹试验检测各组药物血清对增殖后的肝星状细胞JAK2、STAT3蛋白的表达水平。结果:加味四逆散作用6h后,JAK2、STAT3蛋白的表达水平开始降低,且JAK2降低较为明显,24h STAT3降低最为明显。加味四逆散各拆方组作用12h后,STAT3蛋白水平降低,且存在差异。结论:(1)加味四逆散全方及拆方均有降低增殖后的肝星状细胞JAK2、STAT3蛋白的表达。(2)加味四逆散全方阻断JAK2-STAT3通路信号转导的作用显著优于拆方的效果,综合运用比拆方有更好疗效。
OBJECTIVE: To observe the effects of Jiawei Sini Decoction and its demotic prescription serum on JAK2-STAT3 signaling pathway in hepatic stellate cells (HSC-T6). Methods: The normal Wistar rats were gavaged with Jiawei Sini San and decoction decoction for 7 days to prepare serum containing drugs; cultured hepatic stellate cells; stimulated by 100ng / mL leptin to proliferate HSC-T6; Western blotting assay was used to detect the expression of JAK2 and STAT3 protein in proliferating hepatic stellate cells. Results: The JAK2 and STAT3 protein expression began to decrease after Jixin Sini San for 6 hours, and the JAK2 decreased more obviously and the decrease of STAT3 in 24h was the most obvious. After 12 hours’ action of Jiawei Sini Decoction, the STAT3 protein level decreased and there were differences. Conclusion: (1) Jiawei Sini Decoction and its demolition all reduced the expression of JAK2 and STAT3 in proliferating hepatic stellate cells. (2) The effect of Jiawei Sini San on blocking the signal transduction of JAK2-STAT3 pathway was significantly better than that of demolition, and the comprehensive use of the JAK2-STAT3 pathway had better efficacy than that of the demolition side.