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首次采用模式生物斑马鱼研究续断中主要活性成分川续断皂苷VI的代谢,探索斑马鱼用于微量中药皂苷类成分代谢的可行性及合理性。采用高效液相色谱电喷雾质谱检测,Zorbax Extend C18(150 mm×4.6 mm,5μm)色谱柱,0.05%甲酸乙腈0.05%甲酸水梯度洗脱,正、负离子模式同时检测,根据准分子离子峰获得化合物分子量信息,通过与文献数据或对照品对照,鉴定川续断皂苷VI经斑马鱼暴露24 h后的药液及鱼体中的代谢产物。结果川续断皂苷VI经斑马鱼作用后,产生了脱去1分子、2分子及全部糖基的5种脱糖基代谢产物和1个羟基化产物,这与川续断皂苷VI在大鼠体内的代谢机制一致,提示斑马鱼对川续断皂苷VI的代谢具合理性,更具有化合物用量少,成本低、简单、高效的优势,特别是能体现在体代谢的综合结果,有望成为快速预测少量中药成分代谢、补充现有模型不足的全新的、模式生物代谢模型。
For the first time, the metabolism of the main active ingredient Chuan Ssu-sai saponin VI in the model organism zebrafish study was explored to explore the feasibility and rationality of zebrafish for the metabolism of the trace components of saponins in traditional Chinese medicine. High performance liquid chromatography-electrospray ionization mass spectrometry (ESI-MS / MS) was performed on a Zorbax Extend C18 column (150 mm × 4.6 mm, 5 μm) with a gradient of 0.05% formic acid and 0.05% formic acid. The positive and negative ion modes were simultaneously detected. Compound molecular weight information, through the comparison with the literature data or reference substance, identified Duan Duan saponins VI after 24 h exposure to zebrafish liquid and fish metabolites. Results After dipsacus saponin VI was zebrafish, five kinds of deglycosylated metabolites and one hydroxylated product were obtained, which were removed one molecule, two molecules and all the glycosyl groups, The metabolic mechanism in vivo is consistent, suggesting that zebrafish has the rationality of metabolism of Dictyosaponin-D, and has the advantages of less dosage, low cost, simple and high efficiency, especially the comprehensive result of body metabolism, which is expected to be rapid Predict the metabolism of a small amount of traditional Chinese medicine ingredients to supplement the new model metabolic model of the existing models.