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油菜黑胫病是由弱毒种Leptosphaeria biglobosa和强毒种L.maculans混合感染引起的油菜严重病害.本文比较研究了弱毒种预接种、化学诱导剂水杨酸类似物(acibenzolar-S-methyl)和维他命K3类似物(menadione sodium bisulphite)处理所诱导的油菜对强毒种在抗性表型、组织细胞和RNA水平上的反应.诱导接种或化学处理后用强毒种接种.强毒种接种后8~21d,受诱导的植株其病斑扩展速度比水处理的对照显著降低,无论是受诱导的叶(局部效应)还是上部未诱导的叶(系统效应)均如此.强毒种接种后96h,观察到侵入叶组织的病原菌菌丝周围坏死的叶肉细胞,而在对照的植株上没有观测到.定量PCR分析表明,病害防御反应的标记基因PDF1.2,PR-1,NPR1,APX和CHB4在接种后0~96h的表达发生了变化.弱毒种预接种处理优先激活了茉莉酸/乙烯途径的基因表达,包括NPR1的诱导加强表达,这种效应不仅是局部的,而且是系统的.
Brassica campestris is a serious disease of rapeseed caused by the mixed infection of Leptosphaeria biglobosa and L.maculans, a virulent species.This study compared the effects of pre-inoculation, chemical inducer acibenzolar-S-methyl and Response of induced rape to virulent species at resistant phenotypes, tissue cells and RNA levels induced by menadione sodium bisulphite treatment Inoculation or chemical treatment was followed by inoculation with virulent species. After virulent species inoculation From 8 to 21 days, the lesion expansion rate of the induced plants was significantly lower than that of the water-treated control, both induced leaf (topical effect) and upper uninduced leaf (systemic effect) , Necrotic mesophyll cells around the mycelium of pathogenic bacteria invaded into the leaf tissue were observed but not in the control plants.Quantitative PCR analysis showed that the marker genes for disease defense response PDF1.2, PR-1, NPR1, APX and CHB4 The expression changed from 0 to 96 h after inoculation.Pre-inoculation of attenuated strains preferentially activated the gene expression of jasmonic acid / ethylene pathway, including the enhanced expression of NPR1, which is not only localized but also the effect of of.