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目的探讨胃癌组织中miR-148a基因甲基化与临床病理特征、DNA甲基转移酶1(DNA methyltransferase 1,DNMT1)表达的关系。方法 40例胃癌患者的胃癌组织(胃癌组)及其癌旁组织(癌旁组),采用荧光定量PCR法检测2组miR-148a表达情况,采用甲基化特异性PCR检测miR-148a甲基化状态,采用免疫组织化学法检测DNMT1表达情况。结果胃癌组miR-148a表达水平明显低于癌旁组(P<0.01),胃癌组miR-148a甲基化率为70.0%,癌旁组为17.5%,2组比较差异有统计学意义(P<0.05),胃癌组miR-148a甲基化状态在肿瘤大小、浸润深度上差异有统计学意义(P<0.05);胃癌组DNMT1蛋白阳性率为72.5%,癌旁组为27.5%,2组比较差异有统计学意义(P<0.05),胃癌组DNMT1阳性率与miR-148a甲基化状态呈正相关(r=0.33,P<0.05)。结论胃癌组织中miR-148a基因呈高度甲基化状态,可能与DNMT1过表达有关。
Objective To investigate the relationship between the methylation of miR-148a gene and clinicopathological features and DNA methyltransferase 1 (DNMT1) expression in gastric cancer. Methods The expression of miR-148a was detected by fluorescence quantitative PCR in 40 cases of gastric cancer (gastric cancer group) and adjacent normal tissues (paracancer group). The methylation-specific PCR was used to detect the expression of miR-148a methyl The status of DNMT1 expression was detected by immunohistochemistry. Results The miR-148a expression level in gastric cancer group was significantly lower than that in paracancer group (P <0.01). The methylation rate of miR-148a in gastric cancer group was 70.0% and that in paracancer group was 17.5%, and the difference was statistically significant <0.05). The methylation status of miR-148a in gastric cancer group was significantly different between tumor size and depth of invasion (P <0.05) The difference was statistically significant (P <0.05). The positive rate of DNMT1 in gastric cancer group was positively correlated with the methylation status of miR-148a (r = 0.33, P <0.05). Conclusion The miR-148a gene in gastric cancer is highly methylated, which may be related to the overexpression of DNMT1.