论文部分内容阅读
A strain isolated from the fruiting body of a fungus parasitized on Elaphomyces was identified as Cordyceps ophioglossoides based on the morphological characteristics and the analysis of ITS-5.8s rDNA sequence.The optimal medium composition(g·L-1),containing sucrose 66.0,yeast powder 10.0,silkworm chrysalises digest 30.0,MgSO4·7H2O 0.4,and KH2PO4 0.4,was found using fractional factorial design and a central composite design,and the optimization of cultural conditions obtained a result of seed age 6 days,inoculum size 6%(by volume),initial pH 5.6,temperature 24°C,shaking speed 160 r·min-1 by one-factor-at-a-time method.The maximum biomass reached about 20.2 g·L-1 after 90 hours culture under the optimal conditions.Elementary pharmacological activities showed that mycelia of C.ophioglossoides L2 from submerged culture promoted uterus growth in estrogendepleted mice.In the 15-litre scale-up fermentation,the mycelial biomass was around 19.1 g·L-1,indicating a promising prospect for this biotechnology and the potency to develop its medical value.
A strain isolated from the fruiting body of a fungus parasitized on Elaphomyces was identified as Cordyceps ophioglossoides based on the morphological characteristics and the analysis of ITS-5.8s rDNA sequence. The optimal medium composition (g · L-1), containing sucrose 66.0, yeast powder 10.0, silkworm chrysalises digest 30.0, MgSO4.7H2O 0.4, and KH2PO4 0.4, was found using fractional factorial design and a central composite design, and the optimization of cultural conditions obtained a result of seed age 6 days, inoculum size 6% ( by volume), initial pH 5.6, temperature 24 ° C, shaking speed 160 r · min -1 by one-factor-at-a-time method. The maximum biomass reached about 20.2 g · L -1 after 90 hours culture under the Optimal conditions. Elemental pharmacological activities showed that mycelia of C.ophioglossoides L2 from submerged culture promoted uterus growth in estrogen depleted mice. In the 15-liter scale-up fermentation, the mycelial biomass was around 19.1 g · L -1, indicating a promising prospect for t his biotechnology and the potency to develop its medical value.