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采用RE-HPLC方法测定生、炙百部分别与黄芩配伍后化学成分含量进行比较。流动相为甲醇-水-磷酸(47∶53∶0.2),流速:1.0 mL·min-1,检测波长:280 nm。生、炙百部分别与黄芩配伍后黄芩苷与其它成分分离度良好。高效液相色谱法(HPLC)测定黄芩苷,可达线分离,黄芩苷对照品进样量在0.069~0.299μg范围内呈线性关系,R=0.9997,平均回收率为96.93%,RSD=1.5%(n=6)。表明该方法简便快捷,结果准确,重现性好,生方中平均含量为47.5 mg·g-1;炙方中平均含量为60.0 mg·g-1。
The content of chemical constituents after compatibility with Radix Astragali and Radix Astragali was determined by RE-HPLC method. The mobile phase was methanol-water-phosphoric acid (47∶53:0.2), flow rate: 1.0 mL·min-1, detection wavelength: 280 nm. After the compatibility of Radix Astragali and Radix Astragali with Radix Astragali and Radix Astragali, the separation of baicalin and other components was good. High-performance liquid chromatography (HPLC) was used to determine baicalin and reachable line separation. The content of reference substance for baicalin was linear in the range of 0.069-0.299 μg, R=0.9997. The average recovery was 96.93%, and RSD was 1.5%. (n=6). The results showed that the method was simple and rapid, the results were accurate, and the reproducibility was good. The average content in the raw prescription was 47.5 mg·g-1, and the average content in the prescription was 60.0 mg·g-1.