预处理对巴西盾柱木种子吸涨、活力测试及其加速老化的影响(英文)

来源 :Journal of Forestry Research | 被引量 : 0次 | 上传用户:rambo0316
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对巴西盾柱木(Peltophorumdubium)种子进行了4种处理的吸胀实验,即将种子分别置于10℃和27℃下0.2%Captan溶液中和-1.0MPa的PEG6000溶液中,每个处理有四个重复,40粒种子,置于培养皿中浸满实验溶液的双层过滤纸上,覆以透气良好的薄膜。种子在实验前均用98%的浓硫酸处理15分钟以打破机械休眠。吸胀曲线显示,种子在吸胀过程中最终重量的增加值为70%-150%,以后进入一个停滞期。在5种处理下(对照组,PEG6000溶液中10℃和27℃下预处理,Captan溶液中10℃和27℃下预处理),对种子萌发所受到的影响进行了实验。每一个处理分别进行3个次级处理,即在实验前,将种子用蒸馏水分别浸泡12、24和36小时。萌发率最高的是经过蒸馏水浸泡12小时对照组种子和PEG27℃组的种子,萌发率达到100%;萌发率最低的是蒸馏水中浸泡36小时的PEG27℃组的种子,萌发率为52%;经过蒸馏水浸泡24小时的PEG10℃组种子的平均萌发时间为1.08天。蒸馏水中浸泡12小时的PEG27℃组种子平均萌发时间为2.42天,其它处理的萌发时间值介于两者之间。对预处理和未经预处理种子通过加速老化实验,对其活力和生存力进行了测试。经72小时加速老化,种子萌发率低或没有萌发力。对照组种子在培养皿(27℃)和室温条件下蛭石中的萌发率都比处理组高,表现了对老化更强的抗性 Four experiments of swelling experiments on Peltophorum dubium seeds of Brazil were carried out by placing the seeds in 0.2% Captan solution at 10 ° C and 27 ° C and -1.0 MPa in PEG6000 solution with four Repeat, 40 seeds, placed in Petri dishes double-coated filter paper impregnated with the test solution, covered with a well-ventilated film. The seeds were treated with 98% concentrated sulfuric acid for 15 minutes before the experiment to break the mechanical hibernation. The imbibition curve shows that the final weight increase of seeds during imbibition is 70% -150%, and then enters a stagnant period. The effect of seed germination was tested under 5 treatments (control, PEG6000 pretreatment at 10 ° C and 27 ° C, Captan solution pretreatment at 10 ° C and 27 ° C). Three sub-treatments were performed for each treatment, ie, the seeds were soaked in distilled water for 12, 24 and 36 hours before the experiment. The germination rate was 100% in the seeds of the control group and the group of PEG27 ℃ after being soaked for 12 hours in distilled water. The lowest germination rate was in the group of PEG27 ℃ for 36 hours immersed in distilled water with the germination rate of 52% The mean germination time of PEG10 ℃ seeds immersed in distilled water for 24 hours was 1.08 days. The average germination time of PEG27 ℃ seeds immersed in distilled water for 12 hours was 2.42 days, and the germination time of other treatments was between the two. Their viability and viability were tested by accelerated aging experiments on pre-treated and non-pre-treated seeds. After 72 hours of accelerated aging, seed germination rate is low or no germination force. The germination rate of the control group seeds in vermiculite was higher in the petri dish (27 ° C) and at room temperature than the treatment group, showing stronger resistance to aging
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