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目的研究RASSF1A基因启动子的甲基化状态及在膀胱癌和肾癌发生中的作用。方法采用甲基化特异性PCR(MSP)技术检测45例手术切除的膀胱癌组织和相应癌旁正常组织标本、9例电切膀胱癌组织、3例非肿瘤患者正常膀胱组织、12例肾癌组织和相应癌旁正常组织标本中RASSF1A基因的甲基化情况。结果膀胱癌组织中RASSF1A基因异常甲基化55.6%(30/54),其中手术切除组57.8%(26/45),电切癌组织异常甲基化4例,癌旁正常组织基因异常甲基化1例;肾癌组织中基因异常甲基化66.7%(8/12),相应癌旁正常组织中未发现基因甲基化。3例正常膀胱组织标本中未发现基因异常甲基化。膀胱癌组织中RASSF1A基因甲基化率有随组织分期升高的趋势,但与临床病理参数间无明显相关性。结论膀胱癌和肾癌组织中RASSF1A基因启动子高度甲基化,表明该基因甲基化可能与2种肿瘤的发生相关。
Objective To investigate the methylation status of RASSF1A promoter and its role in the development of bladder and renal cell carcinoma. Methods The methylation specific PCR (MSP) technique was used to detect 45 cases of resected bladder cancer tissues and corresponding normal tissues, 9 cases of resected bladder cancer tissues, 3 cases of normal bladder tissues of non-tumor patients and 12 cases of renal cancer Methylation of RASSF1A Gene in Tissues and Corresponding Normal Paracancerous Tissues. Results The abnormal methylation of RASSF1A gene was found in 55.6% (30/54) of the bladder cancer tissues, including 57.8% (26/45) in the resection group, 4 cases of abnormal methylation in the resected cancer tissues and abnormal methyl 1 case of renal cell carcinoma; abnormal methylation of 66.7% (8/12) in renal cell carcinoma tissues, and no methylation of gene in corresponding normal tissues. No abnormal gene methylation was found in 3 cases of normal bladder tissue. The methylation rate of RASSF1A gene in bladder cancer tissues tended to increase with the stage of tissue, but there was no significant correlation with clinicopathological parameters. Conclusion The promoter methylation of RASSF1A gene in bladder cancer and renal cell carcinoma is highly methylated, which indicates that the methylation of RASSF1A gene may be related to the occurrence of two kinds of tumors.