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目的探讨人类抗砷相关基因hARRGcDNA对砷化物的抵抗作用。方法将人类抗砷相关基因hARRGcDNA基因开放阅读框克隆于真核表达载体pcDNA4/HisC中。通过磷酸钙共沉淀方法将带hARRGcDNA基因开放阅读框的表达质粒与空pcDNA4/HisC质粒分别转染到人正常肝L鄄02细胞中,细胞分别用2、4、6、8、10μmol/LNaAsO2染毒,根据染砷细胞存活数确定hARRGcDNA的抗砷性。结果生物信息学分析hARRG蛋白为膜外蛋白,转染含hARRGcDNA基因开放阅读框的pcDNA4/HisC质粒在砷环境中细胞存活数目多于未带hARRG基因开放阅读框的空质粒。结论hARRGcDNA基因具有一定的抗砷作用。
Objective To investigate the resistance of human anti-arsenic related gene hARRG cDNA to arsenic. Methods The human anti-arsenic related gene hARRG cDNA was cloned into the eukaryotic expression vector pcDNA4 / HisC. The expression plasmid with hARRG cDNA open reading frame was transfected into human normal liver L-02 cells by calcium phosphate coprecipitation method. The cells were transfected with 2,4,6,8,10 and 10μmol / L AsAsO2 According to the arsenic cell survival, the aAR resistance of hARRG cDNA was determined. Results Bioinformatics analysis revealed that the hARRG protein was an extramembrane protein and the number of pcDNA4 / HisC plasmids transfected with the open reading frame of hARRG cDNA gene was higher than that of the aa without hARRG gene open reading frame in arsenic environment. Conclusion hARRG cDNA has a certain anti-arsenic effect.