A laser-induced resonance light scattering (RLS) imaging method to determine heparin is described based on the high light scattering emission power of the aggregation species of heparin with α, β, γ, δtetra(4-trimethylaminoniumphenyl)prophyrin (TAPP) in solution. By imaging the light scattering signals of the aggregation species, we proposed the method to determine the heparin with a detection range of 0.02 - 0.6 μg/mL and the detection limit (3 σ) of 1.3 ng/mL.