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目的:研究miRNA-96在结直肠癌转移中的作用及其机制。方法:采用Transwell试验分析结直肠癌Lo Vo细胞的迁移和侵袭能力,采用荧光素报告基因及蛋白免疫印迹试验研究结直肠癌中miR-96的作用靶点。结果:miR-96抑制剂处理后下调miR-96的表达并抑制Lo Vo细胞的迁移和侵袭。荧光素报告基因试验显示RECK是miR-96的作用靶点,且RECK沉默能够部分阻碍miR-96抑制剂所导致的Lo Vo细胞迁移和侵袭减少。结论:miRNA-96可通过作用于RECK促进结直肠癌细胞转移,这可能成为治疗结直肠癌转移的新靶点。
Objective: To investigate the role of miRNA-96 in metastasis of colorectal cancer and its mechanism. Methods: Transwell assay was used to analyze the migration and invasion ability of colorectal cancer LoVo cells. Fluorescein reporter gene and Western blotting were used to study the role of miR-96 in colorectal cancer. Results: The miR-96 inhibitor downregulated the expression of miR-96 and inhibited the migration and invasion of LoVo cells. Fluorescein reporter assay showed that RECK is the target of miR-96, and RECK silencing can partially block the decrease of LoVo cell migration and invasion induced by miR-96 inhibitor. Conclusion: miRNA-96 can promote the metastasis of colorectal cancer cells by acting on RECK, which may be a new target for the treatment of colorectal cancer metastasis.