论文部分内容阅读
选用马铃薯‘Favorita’品种,采用淹水及淹水后恢复通气的方法,研究了缺氧及氧恢复条件对马铃薯块茎亚细胞水平线粒体中活性氧(ROS)及抗氧化酶的影响。结果表明:缺氧胁迫会导致块茎线粒体中超氧阴离子(O2ˉ·)的发生速率、过氧化氢(H2O2)及丙二醛(MDA)的含量先升高后下降,其中在缺氧第1天时分别比对照升高43.95%、49.18%、69.20%,在缺氧第3天时各项指标均达到最大值;而缺氧胁迫下超氧化物歧化酶(SOD)、抗坏血酸过氧化物酶(APX)、过氧化氢酶(CAT)活性呈现先降低、后升高、再降低的趋势,其中缺氧第1天分别比对照降低28.35%、31.48%、37.36%。氧恢复时O2ˉ·发生速率,H2O2及MDA含量,SOD、APX、CAT的活性同样呈现先升高后降低的趋势,其中与缺氧1 d未恢复对照相比,氧恢复1 d分别提高144.69%、63.45%、59.07%、39.29%、11.45%、2.88%。另外,上述各项指标因缺氧胁迫与氧恢复时间的不同而有较大的变化。由此推测:氧恢复比缺氧胁迫更能促进马铃薯块茎线粒体ROS的爆发,加剧膜脂过氧化,并增强线粒体抗氧化酶的短时防御性能。
The selection of ’Favorita’ potato varieties was conducted to study the effects of hypoxia and oxygen recovery conditions on reactive oxygen species (ROS) and antioxidant enzymes in mitochondria of potato tubers at sub-cellular level. The results showed that: hypoxia stress could lead to the occurrence of superoxide anion (O2ˉ ·) in tuber mitochondria, the content of H2O2 and MDA increased first and then decreased, and on the first day of hypoxia, Which was 43.95%, 49.18% and 69.20% higher than that of the control, respectively, and reached the maximum at the third day of hypoxia. Under the condition of hypoxia, the activities of superoxide dismutase (SOD), ascorbate peroxidase (APX) CAT activity decreased first, then increased and then decreased. The first day after hypoxia decreased 28.35%, 31.48% and 37.36% respectively compared with the control. Oxygen recovery rate of O2ˉ, H2O2 and MDA content, SOD, APX, CAT activity also showed a trend of first increased and then decreased, with 1 d did not recover compared with control, oxygen recovery 1 d increased by 144.69% , 63.45%, 59.07%, 39.29%, 11.45%, 2.88%. In addition, the above indicators due to hypoxia stress and oxygen recovery time have a greater change. It was speculated that oxygen recovery could promote ROS burst in potato tuber more than hypoxia stress, aggravate membrane lipid peroxidation and enhance short-term defense of mitochondrial antioxidant enzymes.