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目的 探讨非甾体类 5α 还原酶抑制剂DQI对大运动量游泳训练大鼠血清雄激素水平、EPO水平及肾脏组织EPO合成能力的影响。方法 以渐增游泳训练为大鼠运动应激模型 ,运用酶免和放免分析法观察了血睾、血清EPO及血清 5α 双氢睾酮的含量 ,以及运用RT PCR法观察了肾脏组织EPOmRNA的表达水平。结果 本运动模型可提高靶组织睾酮向 5α 双氢睾酮的转化 ,而DQI可以显著抑制这一过程 ,并可以提高肾脏组织EPO的合成能力及血清EPO含量。结论 非甾体类 5α 还原酶抑制剂DQI对维持及恢复运动能力有一定作用。其机制与整合内源性睾酮及增加内源性EPO有关
Objective To investigate the effects of DQI, a non-steroidal 5α-reductase inhibitor, on serum androgen levels, EPO level and EPO synthesis capacity in kidney tissue of trained rats. Methods With increasing swimming training as the model of exercise stress in rats, the content of blood testosterone, serum EPO and serum 5α dihydrotestosterone were observed by enzyme immunoassay and radioimmunoassay, and the expression of EPO mRNA in renal tissues was observed by RT PCR . Results The model of exercise could improve the conversion of testosterone to 5α dihydrotestosterone in target tissues. However, DQI could significantly inhibit this process and increase the synthesis of EPO and the level of EPO in renal tissues. Conclusion DQI, a non-steroidal 5α-reductase inhibitor, may play a role in maintaining and restoring motor function. Its mechanism is related to the integration of endogenous testosterone and increased endogenous EPO