High-speed cameras are widely used in experimental research and industrial measurement. Although multiple cameras are commonly used, whether the cameras are triggered at the same time is typically overlooked. This study measures the startup time difference between two high-speed cameras employing a proposed measuring system. A series of comparative experiments was conducted to consider the complex factors that can lead to a time difference. The system recorded the startup time differences for different combinations of two cameras at different frame rates, and thus acquired the dependence of the time difference on these factors. Suggestions are made on the basis of the experimental results.