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目的:探讨不同浓度辛伐他汀对体外培养人正常牙髓细胞增殖及LPS诱导的人牙髓细胞的影响,为寻求最佳影响浓度提供参考依据。方法:以改良组织块法体外获得牙髓细胞,选择不同浓度级别的辛伐他汀4组(0.001,0.01,0.1,1μmol·L-1)作用于细胞,与空白对照组相比,观察能促进人正常牙髓细胞增殖的浓度;进一步细分同一级别的辛伐他汀浓度范围(0.005,0.01,0.015,0.05μmol·L-1)作用于人正常牙髓细胞及脂多糖诱导的人牙髓细胞。结果:高浓度(1μmol·L-1)辛伐他汀抑制人正常牙髓细胞增殖,与对照组相比有统计学意义。而0.01μmol·L-1组辛伐他汀促进人正常牙髓细胞增殖,与对照组相比有统计学意义。从第1天起,0.005,0.01,0.015μmol·L-1及0.05μmol·L-1组辛伐他汀抑制脂多糖诱导的人牙髓细胞增殖;而0.01μmol·L-1抑制效果最明显。结论:辛伐他汀对人牙髓细胞增殖的影响与其浓度有关。高浓度1μmol·L-1则抑制人正常牙髓细胞增殖,0.01μmol·L-1辛伐他汀促进人正常牙髓细胞增殖,而抑制脂多糖诱导的人牙髓细胞增殖。
OBJECTIVE: To investigate the effects of different concentrations of simvastatin on the proliferation of human dental pulp cells and the human dental pulp cells induced by LPS in vitro, so as to provide a reference for seeking the optimal concentration. Methods: Dental pulp cells were obtained in vitro by modified tissue block method. Four different concentrations of simvastatin (0.001, 0.01, 0.1, 1 μmol·L-1) were chosen to induce cell proliferation. Compared with the blank control group, Human normal dental pulp cell proliferation; further subdivided the same level of simvastatin concentration range (0.005,0.01,0.015,0.05μmol·L-1) on human normal pulp cells and lipopolysaccharide-induced human dental pulp cells . Results: Simvastatin at a high concentration (1μmol·L-1) inhibited the proliferation of normal human dental pulp cells, which was statistically significant compared with the control group. 0.01μmol·L-1 group simvastatin promote human normal dental pulp cell proliferation, compared with the control group was statistically significant. Simvastatin 0.005, 0.01, 0.015μmol·L-1 and 0.05μmol·L-1 from the first day inhibited the proliferation of human dental pulp cells induced by lipopolysaccharide, while the inhibitory effect of 0.01μmol·L-1 was the most obvious. Conclusion: The effect of simvastatin on the proliferation of human dental pulp cells is related to its concentration. High concentration 1μmol·L-1 inhibited the proliferation of human dental pulp cells. 0.01μmol·L-1 simvastatin could promote the proliferation of human normal dental pulp cells and inhibit the proliferation of human dental pulp cells induced by lipopolysaccharide.