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目的:研究趋化因子受体(CXCR4)在肾透明细胞癌(CCRCC)中的表达及临床意义。方法:应用免疫组织化学SP法从蛋白水平检测42例CCRCC和10例正常肾组织CXCR4的表达,应用半定量RT-PCR从基因水平检测CXCR4mRNA的表达情况,并分析它们与病理分级、临床分期及淋巴转移等的关系。结果:免疫组织化学法检测表明,CXCR4在CCRCC组织的阳性表达率为78.5%,正常肾组织中未检测到CXCR4蛋白的表达,差异有统计学意义,χ2=18.249,P=0.000。42例CCRCC均有不同程度的CXCR4mRNA表达,35例CXCR4mRNA呈高表达,高表达率为83.3%。CXCR4表达与不同临床分期、病理分级及淋巴转移有关,P<0.05;CXCR4mRNA的表达与病理分级、淋巴转移呈正相关,P<0.05。结论:CXCR4在CCRCC中的高表达可能对肾癌的发生、发展起促进作用,CXCR4可能作为肾癌预后的重要参考指标。
Objective: To study the expression and clinical significance of chemokine receptor (CXCR4) in renal clear cell carcinoma (CCRCC). Methods: The expression of CXCR4 in 42 cases of CCRCC and 10 cases of normal kidney tissue was detected by immunohistochemical SP method. The expression of CXCR4 mRNA was detected by semi-quantitative RT-PCR at the gene level, and their relationship with pathological grade, clinical stage, Lymph node metastasis and other relations. Results: Immunohistochemistry showed that the positive expression rate of CXCR4 in CCRCC tissues was 78.5%, while the expression of CXCR4 protein in normal kidney tissues was not detected (χ2 = 18.249, P = 0.000.42) There were different degrees of CXCR4 mRNA expression, 35 cases of CXCR4 mRNA was highly expressed, the high expression rate was 83.3%. The expression of CXCR4 was correlated with different clinical stages, pathological grade and lymphatic metastasis (P <0.05). The expression of CXCR4 was positively correlated with pathological grade and lymphatic metastasis (P <0.05). Conclusion: The high expression of CXCR4 in CCRCC may promote the occurrence and development of renal cell carcinoma, and CXCR4 may serve as an important reference for the prognosis of renal cell carcinoma.