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目的:研究亚低温治疗对脑出血后大鼠Toll样受体4(toll-like receptor 4,TLR4)/核因子-κB(nuclear factor kappa B,NF-κB)介导的神经炎症反应的影响。方法:60只成年SD大鼠随机分为假手术组(Sham组)、脑出血模型组(ICH组)和亚低温治疗组(n=20)。采用自体血注入法复制大鼠脑出血模型,并给予亚低温干预。应用Berdson评分标准对各组大鼠进行神经功能评分,放射免疫法检测肿瘤坏死因子α(tumor necrosis factor-alpha,TNF-α)、白介素1β(interleukin-1beta,IL-1β)的含量;免疫组织化学法及免疫印迹法检测TLR4、NF-κB的蛋白表达情况。结果:与Sham组比,模型组大鼠神经功能评分明显增加(P<0.05),TNF-α和IL-1β的含量明显升高(P<0.05),血肿周围脑组织TLR4和NF-κB的表达显著上调(P<0.05)。与模型组相比,亚低温治疗组大鼠神经功能评分显著减少(P<0.05),TNF-α和IL-1β的含量明显降低(P<0.05),TLR4和NF-κB蛋白的表达量显著下调(P<0.05)。结论:亚低温治疗能通过调控TLR4/NF-κB表达,减轻大鼠脑出血后血肿周围脑组织的炎症反应,具有一定的神经保护作用。
Objective: To investigate the effects of mild hypothermia on neuroinflammation induced by toll-like receptor 4 (TLR4) and nuclear factor kappa B (NF-κB) in rats after intracerebral hemorrhage. Methods: Sixty adult SD rats were randomly divided into Sham group, ICH group and mild hypothermia group (n = 20). The model of intracerebral hemorrhage was reproduced by autologous blood injection, and mild hypothermia was given. The scores of neurological function were evaluated by Berdson score. The levels of tumor necrosis factor-alpha (TNF-α) and interleukin-1β (IL-1β) were measured by radioimmunoassay. The protein expression of TLR4 and NF-κB was detected by chemical method and Western blot. Results: Compared with Sham group, the neurological scores of model group were significantly increased (P <0.05), the contents of TNF-α and IL-1β were significantly increased (P <0.05), and the expression of TLR4 and NF-κB The expression was significantly up-regulated (P <0.05). Compared with the model group, the neurological scores of the rats in the mild hypothermia group were significantly decreased (P <0.05), the levels of TNF-α and IL-1β were significantly decreased (P <0.05), and the expressions of TLR4 and NF- Down (P <0.05). Conclusion: Mild hypothermia can protect the brain tissue around the hematoma after cerebral hemorrhage by regulating the expression of TLR4 / NF-κB.