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免疫组织化学染色法是病理解剖学领域里的一种新的研究方法。Sternberger(1970)建立的过氧化物酶抗过氧化物酶法,即PAP法,具有很高的敏感性。1974年Taylor将此法引用于福尔马林固定的石蜡切片获得成功后,使免疫组织化学方法得到真正的推广。此法可对多年前存档的旧蜡块制片进行研究。国外还报道将HE染色的切片脱色,用PAP方法证实组织内存在的生长激素。但国内尚未见这方面的资料。我们将多年前HE染色的旧切片脱色后进行免疫组
Immunohistochemical staining is a new research method in the field of pathological anatomy. The peroxidase-peroxidase method established by Sternberger (1970), the PAP method, is highly sensitive. In 1974 Taylor cited this method in formalin fixed paraffin sections to be successful, the immunohistochemical method has been truly promoted. This method allows for the study of old wax block films that were archived many years ago. Foreign also reported HE staining sections decolorization, using PAP method to confirm the presence of tissue growth hormone. However, this information has not yet been seen in China. We will take many years ago HE stained old sections decolorization after the immunization group