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目的:探讨解毒扶阳方对2型糖尿病(T2DM)模型大鼠炎症因子的调节作用。方法:取雄性大鼠,随机分为正常对照组,高脂高糖模型(模型)组,模型+二甲双胍(降糖)组,模型+解毒扶阳(试药)高、中、低剂量组,共6组,除正常对照组外,其他各组按60 mg·kg-1剂量尾静脉一次性快速注射链脲佐菌素制备动物模型,并于模型制作当日各给药组大鼠分别ig给予药物治疗,每日1次,连续12周。每10 d称量各组动物体重1次;给药最后1 d取血测定空腹血糖;取血清采用酶联免疫吸附分析方法测定血清瘦素(LEP)、胰岛素(INS)、白细胞介素(IL)-1、IL-6、C-反应蛋白(CRP)、肿瘤坏死因子-α(TNF-α)水平,计算胰岛素敏感指数(ISI)。结果:实验结果显示,解毒扶阳方与模型组比较,低剂量组有显著差异。血清LEP与模型组比较,试药3个剂量组均显示有非常显著的差异。ISI与模型对照组比较,3个剂量组均有非常显著性的差异。与模型组比较,中剂量组IL-1具有显著差异,高剂量组具有非常显著性差异。解毒扶阳方对TNF-α含量有降低作用,与模型组比较,高、中、低3个剂量组均有非常显著差异。结果表明,对CRP也有调节作用,与模型组比较,高、中、低3个剂量组均具有非常显著的差异。结论:解毒扶阳方对T2DM所致血糖、LEP、INS、IL-1、IL-6、CRP、TNF-α含量升高,均有一定调节作用,使之降到正常或接近正常水平。提示解毒扶阳方对T2DM所致炎症因子含量升高以及糖代谢紊乱,有调节作用。
Objective: To investigate the regulatory effect of Jiedu Fuyang Recipe on inflammatory factors in type 2 diabetes mellitus (T2DM) rats. Methods: Male rats were randomly divided into normal control group, model group with high fat and high glucose, model with metformin (hypoglycemic group), model with high, medium and low dose detoxification Fuyang (test drug) A total of 6 groups, except for the normal control group, the other groups 60mg · kg-1 dose of intravenous injection of streptozotocin one-time rapid injection of animal models, and on the day of model making each rat were given ig Medication, once daily for 12 weeks. The body weight of each group was weighed once every 10 days. The fasting blood glucose was measured at the final day of dosing. Serum levels of serum leptin (LEP), insulin (INS) and interleukin (IL) were measured by enzyme-linked immunosorbent assay ) -1, IL-6, C-reactive protein (CRP) and tumor necrosis factor-α (TNF-α) were measured. Insulin sensitivity index (ISI) was calculated. Results: The experimental results show that detoxification Fuyang Fang compared with the model group, low-dose group were significantly different. Serum LEP compared with the model group, three doses of the test group showed a very significant difference. ISI compared with the model control group, the three dose groups have very significant differences. Compared with model group, IL-1 in middle-dose group had significant difference, and high-dose group had very significant difference. Detoxification Fuyang Decoction has a reduced effect on TNF-α content, compared with the model group, high, middle and low dose groups have very significant differences. The results showed that there is also a regulatory role of CRP, compared with the model group, high, middle and low dose groups have very significant differences. Conclusion: Jiedu Fuyang Decoction can regulate T2DM-induced blood glucose, LEP, INS, IL-1, IL-6, CRP and TNF-α levels to normal levels or near normal levels. Tip detoxification Fuyang on T2DM-induced inflammatory cytokines and glucose metabolism disorder, a regulatory role.