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核磁共振(NMR)波谱法是一种表征分子结构、组成变化的有效手段,能够得到生物组织中的核酸、蛋白质、脂类和糖类等生物大分子的含量、分子的空间排列和结构特征等信息,可以从分子水平上研究肿瘤与对照组织之间的差别,更深入地揭示肿瘤的发生、发展中产生的各种分子水平变化。文章对9例直肠癌组织及癌旁正常组织进行了核磁共振氢谱的测定。结果表明,直肠正常和癌变组织的核磁共振氢谱存在显著的差异,这可以通过对谱图特征峰区域积分面积相对比值的差别看出来。直肠正常组织核磁共振氢谱中A0.9/A3.0,A1.3/A3.0,A2.0/A3.0,A1.3/A0.9及A4.1/A3.0值较相应的癌组织偏大,直肠癌组织核磁共振氢谱中A3.2/A3.0值较相应正常直肠组织偏大。通过这些差异核磁共振氢谱技术有可能发展成为早期诊断直肠癌组织的一种方法。
Nuclear magnetic resonance (NMR) spectroscopy is an effective means of characterizing molecular structure and composition changes, and can obtain biological macromolecules such as nucleic acids, proteins, lipids and carbohydrates, spatial arrangement of molecules and structural features in biological tissues Information, we can study the differences between tumor and control tissues at the molecular level, and reveal more deeply the changes of various molecular levels in the development of tumors. In this paper, 9 cases of rectal cancer and adjacent normal tissue were determined by 1H NMR. The results showed that there was a significant difference in the 1H NMR spectra of normal rectal and cancerous tissues, as can be seen by the difference in the relative integral of the integrated area of the characteristic peak areas in the rectum. The values of A0.9 / A3.0, A1.3 / A3.0, A2.0 / A3.0, A1.3 / A0.9 and A4.1 / A3.0 in the normal rectal tissue were higher than those in the normal rectal tissue Of the cancer tissue is too large, the rectal cancer tissue nuclear magnetic resonance hydrogen A3.2 / A3.0 value is larger than the corresponding normal rectal tissue. It is possible that these differences in 1H NMR spectroscopy may develop into a method of early diagnosis of rectal cancer.