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牛黄解毒片中清热解毒的主要成分为胆酸盐类,我们对牛黄中的胆酸进行薄层层析定量测定,取得了较满意的结果。一、测定方法1.供试液的制备:分别取各厂家生产的牛黄解毒片若干,刮去糖衣研制细末,精密称定细粉5g,置索氏提取器中加100ml氯仿提取4小时,残渣挥尽溶济后,再加入100ml甲醇提取3小时,得甲醇提取液,浓缩至干,加入95%乙醇4ml溶解备用。2.对照液的制备:精密称取胆酸标准品100mg,置25ml的容量瓶中,加入95%乙醇20ml左右,摇匀溶解,稍停,用上述乙醇加至刻度备用。
The main component of detoxification in Niuhuang Jiedu Tablets is cholate, and we have performed quantitative determination of bile acids in bezoar with satisfactory results. First, the determination method 1. Preparation of the test solution: Take each manufacturer’s several Niuhuangjiedu tablets, scrape off the icing to develop fine, accurately weighed fine powder 5g, set Soxhlet extractor plus 100ml chloroform extraction for 4 hours, After the residue is completely evaporated, 100 ml of methanol is added for extraction for 3 hours to obtain a methanol extract, which is then concentrated to dryness, and 4 ml of 95% ethanol is added for dissolution. 2. Preparation of control solution: Accurately weigh 100 mg of bile acid standard product into a 25 ml volumetric flask and add about 95% ethanol to about 20 ml. Shake well and dissolve. Stop briefly and add the above ethanol to the scale for later use.