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目的 观察儿茶素对阿霉素肾病鼠血浆及肾皮质内皮素 (ET)、一氧化氮 (NO)表达的影响。方法 将 36只SD雌性大鼠随机分成正常组、肾病组、激素组、儿茶素预防组、儿茶素治疗组、儿茶素 +激素联合治疗组共 6组。应用生化法测定 2 4h各组尿蛋白排泄量、尿、血浆及肾局部中NO的浓度 ;应用放免法测定血浆及肾局部中ET的浓度 ;应用免疫组化法测定肾组织中固有细胞细胞增生核抗原 (PCNA)的表达 ;并应用半定量评分法对各组大鼠病理改变进行计量分析。结果 (1)实验末 ,大鼠血浆及肾皮质中NO浓度以肾病组为最低 (13 5± 3 7,2 7 8± 12 4 ) ,其他从低到高依次是儿茶素治疗组 (2 5 3± 5 3,4 2 5± 1 9)、儿茶素预防组 (36 8± 5 3,5 5 5± 15 4 )、激素组 (43 3± 4 0 ,73 6±15 8)、儿茶素 +激素联合治疗组 (5 4 3± 7 1,96 4± 17 2 ) (F =6 2 3,79 4 ,P <0 0 1) ;血浆及肾皮质中ET浓度则以肾病组最高 (5 72± 91,14 93± 2 99) ,其他组从高到低顺序与NO浓度从低到高的顺序相同(F =5 4 8,14 6 8,P <0 0 1) ,以儿茶素 +激素联合治疗组为最低 ;各组与肾病组相比 ,差异均有显著意义。 (2 )各组肾小球系膜细胞、上皮细胞、内皮细胞 ,肾小管上皮细胞 ,血管内皮细胞中PCNA阳性表达率均低于肾病组
Objective To investigate the effects of catechin on the expression of ET and NO in plasma and renal cortex of adriamycin-induced nephropathy rats. Methods Thirty - six SD female rats were randomly divided into normal group, nephrosis group, hormone group, catechin prevention group, catechin treatment group and catechin + hormone combination treatment group. Urinary protein excretion, urinary, plasma and renal NO concentration in each group were measured by biochemical method at 24 hours. The concentrations of ET in plasma and kidney were determined by radioimmunoassay. The expression of innate cell proliferation Nuclear antigen (PCNA) expression; and semi-quantitative scoring method of pathological changes in each group were measured. Results (1) At the end of the experiment, the concentration of NO in rat plasma and renal cortex was the lowest (13 5 ± 3 7, 2 7 8 ± 12 4) in nephropathy group and the lowest in the other group was catechin treatment group (2 5 3 ± 5 3,4 2 5 ± 1 9), catechin prophylaxis group (36 8 ± 5 3,5 5 5 ± 15 4), hormone group (43 3 ± 4 0, 73 6 ± 15 8), Catechin + hormones combined treatment group (543 ± 7196 4 ± 17 2) (F = 6 2 3,79 4, P <0.01); plasma and renal cortex in the concentration of ET in nephropathy group (5 72 ± 91, 14 93 ± 2 99), and the order from high to low in other groups was the same as that in NO from low to high (F = 548,146 8, P <0.01) Catechin + hormone combination therapy group was the lowest; each group compared with the nephropathy group, the difference was significant. (2) The positive expression rates of PCNA in glomerular mesangial cells, epithelial cells, endothelial cells, renal tubular epithelial cells and vascular endothelial cells in each group were lower than those in nephropathy group