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旨在探索应激性行为缺陷（ｓｔｒｅｓｓ－ｉｎｄｕｃｅｄｂｅｈａｒｙｉｏｒｄｅｆｉｃｔＳＩＢＤ）大鼠β－肾上腺素能亚型受体的作用。用同位素配基结合技术测定β－肾上腺素能受体结合力。以１２５Ⅰ－Ｃｙａｎｏｐｉｎｄｏｌｏｌ（１２５ⅠＣＹＰ）作为配基，用ｐｒｏｐｒａｎｏｌｏｌ作为竞争性抑制剂测定总β－肾上腺素能受体结合力；用ＩＣＩ８６，４０６，ＩＣＩ１１８，５５１作为β１，β２－肾上腺素能受体竞争性抑制剂分别测定β１，β２－肾上腺素能亚型受体结合力。实验４８ｈ后大鼠脑海马组织β－肾上腺素能受体的最大数目（Ｂｍａｘ）和解离常数（Ｋｄ）增加，这种变化是由于β１－肾上腺素能受体变化所致。结果提示ＳＩＢＤ大鼠脑海马组织β１－肾上腺素能亚型受体变化可能主要涉及早期的行为变化。
Aimed to explore the role of β-adrenergic subtype receptors in stress-induced behavioral deficiencies in rats. Beta-adrenergic receptor binding was determined using isotope ligand binding techniques. The total β-adrenergic receptor binding was measured using 125Ⅰ-Cyanopindolol (125Ⅰ CYP) as a ligand with propranolol as a competitive inhibitor; ICI86,406, ICI118,551 as β1, β2-adrenergic receptor competing Inhibitors were measured β1, β2-adrenergic subtype receptor binding. The maximum number of β-adrenergic receptors (Bmax) and dissociation constant (Kd) in rat hippocampus increased after 48 hours of experiment, which was due to the change of β1-adrenoceptor. The results suggest that the changes of β1-adrenergic subtype receptors in hippocampal tissue of SIBD rats may mainly involve the early behavioral changes.