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采用D-氨基半乳糖诱发大鼠暴发性肝衰竭(FHF)实验模型,以57只大鼠,分为3组,每组19只,分别给予腹腔注射4ml的胎肝细胞悬液(活细胞率在80%以上,有核细胞数为2.575×10~7/ml)、胞质液(电镜观察不含细胞器,蛋白质含量为25mg/ml)和缓冲液(对照组)。结果显示,治疗后4天与10天的存活率,人胎肝细胞悬液组为73.7%与47.4%,胞质液组为63.2%与42.1%,对照组第4天为5.5%,前两组
The experimental model of fulminant hepatic failure (FHF) induced by D-galactose in rats was divided into 3 groups with 19 rats in each group. Four ml of fetal liver cell suspension was injected intraperitoneally (viable cell rate). Above 80%, the number of nucleated cells was 2.575×10~7/ml, cytoplasm (electron microscope observation without organelles, protein content 25 mg/ml) and buffer (control group). The results showed that the survival rates at 4 and 10 days after treatment were 73.7% and 47.4% in the human fetal liver cell suspension group, 63.2% and 42.1% in the cytoplasmic fluid group, and 5.5% in the control group on the fourth day. group