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目的观察AMD3100对造血干/祖细胞(HSC/HPC)动员作用。方法 C57BL/6小鼠分为两组:实验组皮下注射AMD3100 5mg/kg;对照组皮下注射等体积的生理盐水。分别于0、0.5、1、2、4、8及12h取血,检测外周血白细胞计数、流式细胞术检测KSL细胞及KL细胞。结果用药30min后,实验组白细胞、KSL细胞及KL细胞明显高于用药前及对照组(P<0.05),2h达到高峰,随后逐渐下降,到24h下降到用药前水平。结论 AMD3100对HSC/HPC有一定的动员作用,动员速度快,作用时间短。
Objective To observe the mobilization effect of AMD3100 on hematopoietic stem / progenitor cells (HSC / HPC). Methods C57BL / 6 mice were divided into two groups: experimental group, subcutaneous injection of AMD3100 5mg / kg; control group, subcutaneous injection of equal volume of saline. Peripheral blood leukocyte counts were measured at 0, 0.5, 1, 2, 4, 8 and 12 h respectively. KSL cells and KL cells were detected by flow cytometry. Results After treatment for 30min, leukocytes, KSL cells and KL cells in experimental group were significantly higher than those before treatment and in control group (P <0.05), peaked at 2h, then decreased gradually and dropped to pre-treatment level by 24h. Conclusion AMD3100 has a certain mobilization effect on HSC / HPC with fast mobilization and short acting time.